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Titolo:
Genetic diversity in the Helicobacter pylori cag pathogenicity island and effect on expression of anti-CagA serum antibody in UK patients with dyspepsia
Autore:
Peters, TM; Owen, RJ; Slater, E; Varea, R; Teare, EL; Saverymuttu, S;
Indirizzi:
Cent Publ Hlth Lab, Lab Enter Pathogens, Helicobacter Reference Unit, London NW9 5HT, England Cent Publ Hlth Lab London England NW9 5HT Unit, London NW9 5HT, England Publ Hlth Lab, Chelmsford CM2 0YX, Essex, England Publ Hlth Lab Chelmsford Essex England CM2 0YX rd CM2 0YX, Essex, England Broomfield Hosp, Dept Gastroenterol, Chelmsford CM1 7ET, Essex, England Broomfield Hosp Chelmsford Essex England CM1 7ET CM1 7ET, Essex, England
Titolo Testata:
JOURNAL OF CLINICAL PATHOLOGY
fascicolo: 3, volume: 54, anno: 2001,
pagine: 219 - 223
SICI:
0021-9746(200103)54:3<219:GDITHP>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
VACUOLATING-CYTOTOXIN; GASTRIC-CANCER; VIRULENCE FACTORS; PEPTIC-ULCER; STRAINS; INFECTION; PREVALENCE; DISEASES; PROTEIN; RISK;
Keywords:
Helicobacter pylori; cagA; cag pathogenicity island; CagA antibody assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Owen, RJ Cent Publ Hlth Lab, Lab Enter Pathogens, Helicobacter Reference Unit, 61 Colindale Ave, London NW9 5HT, England Cent Publ Hlth Lab 61 Colindale Ave London England NW9 5HT gland
Citazione:
T.M. Peters et al., "Genetic diversity in the Helicobacter pylori cag pathogenicity island and effect on expression of anti-CagA serum antibody in UK patients with dyspepsia", J CLIN PATH, 54(3), 2001, pp. 219-223

Abstract

Aims-To investigate variation within the cag pathogenicity island (PAI) ofHelicobacter pylori isolated from patients with dyspepsia in mid-Essex, and to evaluate the effect on expression of anti-CagA antibody. Methods-Sixty two isolates of H pylori cultured from gastric biopsies werescreened by specific PCR assays for the presence of cagA and other gene markers (cagD and cagE, and virD4) in the cag PAI. An enzyme linked immunosorbent assay (ELISA) kit (Viva Diagnostica helicobacter p120) was used to test for anti-CagA IgG antibody in matching sera. Isolates were also genotypedby vacuolating cytotoxin polymerase chain reaction (PCR) analysis, and tested for absence of the complete cag PAI (empty site FCR assay). Results-Forty one of the H pylori isolates had a cag PAI containing cagA. One strain had no cagA but other cag PAI loci were present, whereas the remaining 20 strains had no detectable cag PAI markers. Anti-CagA IgG antibodywas detected in 34 sera by the ELISA assay, and when compared with the cagPAI genotype of the infecting strain, accuracy, sensitivity, and specificity were 92%, 87%, and 100%, respectively. The seven discrepant or borderline strains in the ELISA were all vacA sl but differed in other genotypic markers. Conclusions-The cag PAI was widely distributed in H pylori from patients with dyspepsia in mid-Essex who had different gastric pathologies. Infectionwith a strain having an uninterrupted cag PAI was associated with the presence of anti-CagA antibody in most patients. Discrepant ELISA results, mostly for elderly patients with duodenal ulcers, were attributed to cagA associated variation, particularly to the presence of mixed cagA+/cagA- cell variants in the infecting strain population. Tests for anti-CagA serum antibody were unreliable for predicting severity of clinical disease associated with H pylori infection in this series of patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 09:00:02