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Titolo:
Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion
Autore:
Josic, D; Buchacher, A; Jungbauer, A;
Indirizzi:
Octapharma Pharmzeut Prod GesmbH, A-1100 Vienna, Austria Octapharma Pharmzeut Prod GesmbH Vienna Austria A-1100 0 Vienna, Austria Univ Agr Sci, Inst Appl Microbiol, A-1190 Vienna, Austria Univ Agr Sci Vienna Austria A-1190 ppl Microbiol, A-1190 Vienna, Austria
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 752, anno: 2001,
pagine: 191 - 205
SICI:
1387-2273(20010310)752:2<191:MASPFS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE MEMBRANE CHROMATOGRAPHY; ION-EXCHANGE CHROMATOGRAPHY; HYDROPHOBIC-INTERACTION CHROMATOGRAPHY; POROUS AGAROSE BEADS; CLOTTING FACTOR-VIII; LIQUID-CHROMATOGRAPHY; AFFINITY-CHROMATOGRAPHY; CONTINUOUS BEDS; CAPILLARY ELECTROCHROMATOGRAPHY; DIMETHACRYLATE) MONOLITHS;
Keywords:
monoliths; enzymatic conversion; proteins; polynucleotides;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Buchacher, A Octapharma Pharmzeut Prod GesmbH, Oberlaaerstr 235, A-1100 Vienna, Austria Octapharma Pharmzeut Prod GesmbH Oberlaaerstr 235 Vienna Austria A-1100
Citazione:
D. Josic et al., "Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion", J CHROMAT B, 752(2), 2001, pp. 191-205

Abstract

Monoliths are considered as a novel generation of stationary phases. They were applied for capillary electrochromatography and liquid chromatography exploiting every action principle such as ion-exchange, affinity recognition, reversed-phase, and hydrophobic interaction. The fast separation was explained by convective transport of the solutes through the bed. The contribution of this mode of transport is similarly explained as done for the beds packed with particles with gigapores. For monolithic beds, the concept of an ultrashort bed was frequently used. This mode of operation allows very short separation time. In many cases a gradient elution is necessary to achieve separation. Examples of applications for protein and polynucleotide separation performed on monoliths are given. Enzymatic conversion was describedshowing the examples of several immobilzed enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:38:15