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Titolo:
Localized activation of m-calpain in migrating human umbilical vein endothelial cells stimulated by shear stress
Autore:
Ariyoshi, H; Yoshikawa, N; Aono, Y; Tsuji, Y; Ueda, A; Tokunaga, M; Sakon, M; Monden, M;
Indirizzi:
Osaka Univ, Sch Med, Dept Surg 2, Suita, Osaka 565, Japan Osaka Univ Suita Osaka Japan 565 ed, Dept Surg 2, Suita, Osaka 565, Japan
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 1, volume: 81, anno: 2001,
pagine: 184 - 192
SICI:
0730-2312(2001)81:1<184:LAOMIM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CYTOPLASMIC CALCIUM-CONCENTRATION; HUMAN BLOOD-PLATELETS; NEUTRAL PROTEASE; 2 FORMS; CA2+; CLEAVAGE; PURIFICATION; PROTEOLYSIS; CALPASTATIN;
Keywords:
shear stress; [Ca2+](i); m-calpain; pp125FAK; ezrin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Ariyoshi, H Osaka Univ, Sch Med, Dept Surg 2, 2-2 Yamadaoka, Suita, Osaka 565, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 565 saka 565, Japan
Citazione:
H. Ariyoshi et al., "Localized activation of m-calpain in migrating human umbilical vein endothelial cells stimulated by shear stress", J CELL BIOC, 81(1), 2001, pp. 184-192

Abstract

Using a parallel-plate flow-chamber and confocal laser scanning microscopy(CLSM), we studied the mode of cytoskeletal reorganization in migrating HUVECs stimulated by shear stress. Activation of m-calpain associated with a change in the spatial distribution of cytoplasmic ionized Ca2+ concentration ([Ca2+](i)) was studied. Shear stress (10 dyne/cm(2)) caused migration and decrease in the F-actin content of HUVECs. Migrating individual HUVECs showed the lamellipodium formed in the direction of cell migration, in which [Ca2+](i) elevated to 148 +/- 12 nM in a localized fashion. We found the appearance of activated m-calpain in the local area of the migrating HUVECs, which was associated with a decrease in the amounts of pp125FAK and ezrin. The localized rise in [Ca2+](i) might he closely related to morphological change to regulate the direction of cell migration induced by shear stress through localized activation of m-calpain. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 08:04:12