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Titolo:
An essential cytoplasmic domain for the Golgi localization of coiled-coil proteins with a COOH-terminal membrane anchor
Autore:
Misumi, Y; Sohda, M; Tashiro, A; Sato, H; Ikehara, Y;
Indirizzi:
Fukuoka Univ, Sch Med, Dept Biochem, Jonan Ku, Fukuoka 8140180, Japan Fukuoka Univ Fukuoka Japan 8140180 hem, Jonan Ku, Fukuoka 8140180, Japan Kanazawa Univ, Canc Res Inst, Dept Mol Oncol & Virol, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 wa, Ishikawa 9200934, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 9, volume: 276, anno: 2001,
pagine: 6867 - 6873
SICI:
0021-9258(20010302)276:9<6867:AECDFT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; MOLECULAR CHARACTERIZATION; VESICULAR TRANSPORT; SECRETORY PATHWAY; COMPLEX; APPARATUS; NETWORK; SYNTAXIN; IDENTIFICATION; REDISTRIBUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Ikehara, Y Fukuoka Univ, Sch Med, Dept Biochem, Jonan Ku, Fukuoka 8140180,Japan Fukuoka Univ Fukuoka Japan 8140180 Ku, Fukuoka 8140180, Japan
Citazione:
Y. Misumi et al., "An essential cytoplasmic domain for the Golgi localization of coiled-coil proteins with a COOH-terminal membrane anchor", J BIOL CHEM, 276(9), 2001, pp. 6867-6873

Abstract

Giantin is a resident Golgi protein that has an extremely long cytoplasmicdomain (about 370 kDa) and is anchored to the Golgi membrane by the COOH-terminal membrane anchoring domain (CMD) with no luminal extension. We examined the essential domain of giantin required for Golgi localization by mutational analysis. The Gels localization of giantin was not affected by the deletion of its CMD or by substitution with the GRID of syntaxin-2, a plasmamembrane protein. The giantin CMD fused to the cytoplasmic domain of syntaxin-2 could not retain the chimera in the Golgi apparatus. Sequential deletion analysis showed that the COOH-terminal sequence (positions 3059-3161) adjacent to the CMD was the essential domain required for the Golgi localization of giantin. We also examined two other Gels-resident proteins, golgin-84 and syntaxin-5, with a similar membrane topology as giantin. It was confirmed that the cytoplasmic domain of about 100 residues adjacent to the CMDwas required for their Golgi localization. Taken together, these results suggest that the COOH-terminally anchored Golgi proteins with long cytoplasmic extensions have the Golgi localization signal(s) in the cytoplasmic sequence adjacent to the CMD. This is in contrast to previous observations thata transmembrane domain is required for Golgi localization by other Golgi proteins transported from the endoplasmic reticulum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:45:07