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Titolo:
Amyloid beta protein precursor is involved in the growth of human colon carcinoma cell in vitro and in vivo
Autore:
Meng, JY; Kataoka, H; Itoh, H; Koono, M;
Indirizzi:
Miyazaki Med Coll, Dept Pathol 2, Kiyotake, Miyazaki 8891692, Japan Miyazaki Med Coll Kiyotake Miyazaki Japan 8891692 Miyazaki 8891692, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 1, volume: 92, anno: 2001,
pagine: 31 - 39
SICI:
0020-7136(20010401)92:1<31:ABPPII>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-TERMINAL REGION; EXTRACELLULAR-MATRIX; ALZHEIMERS-DISEASE; SECRETED FORM; IN-VIVO; KINASE; CANCER; DIFFERENTIATION; PROLIFERATION; EXPRESSION;
Keywords:
amyloid beta protein precursor; colon carcinoma cell; antisense; growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kataoka, H Miyazaki Med Coll, Dept Pathol 2, 5200 Kihara, Kiyotake, Miyazaki 8891692,Japan Miyazaki Med Coll 5200 Kihara Kiyotake Miyazaki Japan 8891692 n
Citazione:
J.Y. Meng et al., "Amyloid beta protein precursor is involved in the growth of human colon carcinoma cell in vitro and in vivo", INT J CANC, 92(1), 2001, pp. 31-39

Abstract

Amyloid beta protein precursor (APP) is a membrane-bound protein ubiquitously expressed in a variety of types of cells. However, its biological functions remain largely uncertain, particularly in non-neural cells and tumors. Our previous studies revealed that a secreted form of APP having a Kunitz-type inhibitor domain is a major serine proteinase inhibitor secreted by human colon carcinoma cells. In our study, we used an antisense RNA strategy to selectively inhibit the expression of APP in the human colon carcinoma cell line SW837. A vector capable of expressing an antisense mRNA complementary to 911 bases of the 5' end of APP mRNA was transfected into SW837 cells. After selection, 2 stably transfected antisense clones were obtained in which both the APP protein and mRNA were significantly suppressed. The proliferative potential and colony-forming efficiency of the antisense clones invitro were markedly suppressed compared with the parent and mock-transfected clones. The addition of the conditioned medium of parent cells or purified secretory APP enabled these antisense effects to be overcome in vitro. The suppressed growth was also observed in vivo when the cells were injectedsubcutaneously into nude mice. Histologically, formation of tubular structures appeared to be suppressed in the antisense clones in vivo. These observations suggest potentially important roles of APP in cellular proliferation and differentiation of colon carcinoma cells. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 15:17:17