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Titolo:
CD44 binds a chondroitin sulfate proteoglycan, aggrecan
Autore:
Fujimoto, T; Kawashima, H; Tanaka, T; Hirose, M; Toyama-Sorimachi, N; Matsuzawa, Y; Miyasaka, M;
Indirizzi:
Osaka Univ, Grad Sch Med, Biomed Res Ctr, Dept Bioregulat, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 regulat, Suita, Osaka 5650871, Japan Osaka Univ, Grad Sch Med, Dept Internal Med & Mol Sci, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 Mol Sci, Suita, Osaka 5650871, Japan Tokyo Metropolitan Inst Med Sci, Dept Immunol, Bunkyo Ku, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 3, volume: 13, anno: 2001,
pagine: 359 - 366
SICI:
0953-8178(200103)13:3<359:CBACSP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SYNOVIAL-FLUID; CARTILAGE PROTEOGLYCAN; MONOCLONAL-ANTIBODIES; L-SELECTIN; RHEUMATOID-ARTHRITIS; ADHESION MOLECULE; TYROSINE KINASES; CELL ACTIVATION; ADAMTS FAMILY; TUMOR-GROWTH;
Keywords:
aggrecan; CD44; chondroitin sulfate; extracellular matrix; proteoglycan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Miyasaka, M Osaka Univ, Grad Sch Med, Biomed Res Ctr, Dept Bioregulat, 2-2Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
T. Fujimoto et al., "CD44 binds a chondroitin sulfate proteoglycan, aggrecan", INT IMMUNOL, 13(3), 2001, pp. 359-366

Abstract

Here we report that CD44 binds a chondroitin sulfate (CS) proteoglycan, aggrecan, a major component of cartilage, Soluble CD44-IgG and CD44(+) cells bound to aggrecan from rat chondrosarcoma and bovine cartilage, immobilizedon microtiter plates. In both cases, binding was blocked by a neutralizinganti-CD44 mAb or by the pretreatment of aggrecan with chondroitinase, but not hyaluronidase or keratanase, indicating that CD44 binds aggrecan in a manner dependent on CS side chains of aggrecan and that hyaluronic acid is not involved in the binding, Structural analysis showed that glycosaminoglycans of aggrecan from rat chondrosarcoma and bovine articular cartilage consist of mainly CS A and a mixture of CS A and C respectively. When immobilized on microtiter plates, both CS A and C bound CD44-IgG, and the reaction was specifically inhibited by an anti-CD44 mAb, In addition, aggrecan augmented apoptosis in cells expressing CD44-Fas chimeric molecules in synergy with a non-blocking anti-CD44 mAb IRAWB14,4, suggesting that CD44-aggrecan interaction can induce oligomerization of the chimeric molecules, These results suggest that aggrecan interacts with CD44 to mediate cell adhesion and to trigger oligomerization of CD44 molecules, which may lead to intracellular signaling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:41:27