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Titolo:
Overexpression of neural cell adhesion molecule in Chagas' myocarditis
Autore:
Soler, AP; Gilliard, G; Xiong, YM; Knudsen, KA; Martin, JL; De Suarez, C; Gamboa, JDM; Mosca, W; Zoppi, LB;
Indirizzi:
Lankenau Inst Med Res, Wynnewood, PA 19096 USA Lankenau Inst Med Res Wynnewood PA USA 19096 Res, Wynnewood, PA 19096 USA Bryn Mawr Hosp, John S Sharpe Res Fdn, Bryn Mawr, PA USA Bryn Mawr Hosp Bryn Mawr PA USA John S Sharpe Res Fdn, Bryn Mawr, PA USA Cent Univ Venezuela, Sch Med, Dept Cardiovasc Pathol, Caracas, Venezuela Cent Univ Venezuela Caracas Venezuela iovasc Pathol, Caracas, Venezuela Cent Univ Venezuela, Sch Med, Inst Biomed, Dept Physiopathol, Caracas, Venezuela Cent Univ Venezuela Caracas Venezuela Physiopathol, Caracas, Venezuela
Titolo Testata:
HUMAN PATHOLOGY
fascicolo: 2, volume: 32, anno: 2001,
pagine: 149 - 155
SICI:
0046-8177(200102)32:2<149:OONCAM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY EPITOPES; RAT-HEART DEVELOPMENT; N-CAM; TRYPANOSOMA-CRUZI; FUNCTIONAL DETERMINANTS; POLYSIALYLATED NCAM; CADHERIN EXPRESSION; KAPPA-B; MYOFIBRILLOGENESIS; DISEASE;
Keywords:
Chagas' myocarditis; Trypanosoma cruzi; cell adhesion molecules; neural cell adhesion molecule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Soler, AP Lankenau Inst Med Res, 100 Lancaster Ave, Wynnewood, PA 19096 USA Lankenau Inst Med Res 100 Lancaster Ave Wynnewood PA USA 19096 A
Citazione:
A.P. Soler et al., "Overexpression of neural cell adhesion molecule in Chagas' myocarditis", HUMAN PATH, 32(2), 2001, pp. 149-155

Abstract

The expression of the neural cell adhesion molecule (NCAM) was studied in normal human myocardium and in Chagas' disease myocarditis. We found that NCAM is expressed in the conduction system as well as the myocardium in the fetal heart, but its expression is restricted to the conduction system and absent in the adult myocardium. Chagas' disease is an American endemic disease caused by the Trypanosoma cruzi parasite, which produces myocarditis and a blockade of the conduction system, resulting in cardiac dysfunction. Westudied the expression of NCAM in paraffin-embedded human heart tissues from 34 autopsies of patients with Chagas' myocarditis and from murine and canine experimental acute Chagas' myocarditis, using a polyclonal anti-NCAM antibody and immunohistochemistry. Our results show a dramatic upregulation of NCAM expression in the intercalated discs of cardiomyocytes in acute andchronic Chagas' myocarditis, Surprisingly, the NCAM signal was detected inintracellular nests of amastigote forms of the parasite, within infected cardiomyocytes of human and experimental Chagas' myocarditis. In contrast, cardiac cell-cell adhesion proteins, N-cadherin and beta -catenin, were found in intercalated discs distorted by the infection but absent from the amastigote nests. Proteins reactive to several antibodies against NCAM were detected by Western immunoblotting in cultured T cruzi parasites and in trypomastigote forms of T cruzi extracted from the blood of infected mice. The upregulation of NCAM in Chagas' myocarditis and the expression of NCAM or a NCAM-like protein by T cruzi suggest that NCAM may act as a receptor for tissue targeting and cellular invasion by T cruzi in Chagas' disease. HUM PATHOL 32:149-155. Copyright (C) 2001 by W.B. Saunders Company.

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Documento generato il 04/12/20 alle ore 13:26:23