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Titolo:
Detection and measurement of in vitro gene transfer by gamma camera imaging
Autore:
Zinn, KR; Chaudhuri, TR; Buchsbaum, DJ; Mountz, JM; Rogers, BE;
Indirizzi:
Univ Alabama, Dept Radiol, BDB 11, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ol, BDB 11, Birmingham, AL 35294 USA Univ Alabama, Dept Radiat Oncol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 diat Oncol, Birmingham, AL 35294 USA
Titolo Testata:
GENE THERAPY
fascicolo: 4, volume: 8, anno: 2001,
pagine: 291 - 299
SICI:
0969-7128(200102)8:4<291:DAMOIV>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; POSITRON-EMISSION-TOMOGRAPHY; RECEPTOR-BINDING PEPTIDES; IN-VIVO; REPORTER GENE; ADENOVIRAL VECTOR; LIVING ANIMALS; EXPRESSION; THERAPY; PROLIFERATION;
Keywords:
imaging; gene transfer; somatostatin receptor; Tc-99m;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Zinn, KR Univ Alabama, Dept Radiol, BDB 11, 1530 3rd Ave S, Birmingham, AL35294 USA Univ Alabama 1530 3rd Ave S Birmingham AL USA 35294 AL 35294 USA
Citazione:
K.R. Zinn et al., "Detection and measurement of in vitro gene transfer by gamma camera imaging", GENE THER, 8(4), 2001, pp. 291-299

Abstract

The purpose of this work was to develop a high capacity method to image gene transfer to cancer cells growing as monolayers in cell culture plates. Asensitive and high capacity nuclear-imaging method for detection of gene transfer in vitro will allow rapid validation of vectors in different cell lines under various conditions. Human cancer cell lines (A-427 non-small cell lung, SKOV3.ip1 ovarian, MDA-MB-468 breast, and BxPC-3 pancreatic) were infected with a replication-incompetent adenoviral vector encoding the humantype 2 somatostatin receptor (Ad-hSSTr2). Expression of the hSSTr2 reporter protein in cells was detected by imaging an internalized Tc-99m-labeled, hSSTr2 binding peptide (P2045, Diatide, Inc.). Imaging provided an accuratemeasure of internally bound Tc-99m as evidenced by equivalence of results for imaging region of interest (ROI) analyses and gamma counter measurements. Internally bound Tc-99m-P2045 was linearly correlated (R-2 = 0.98) with the percentage of hSSTr2-positive cells following gene transfer. Excess P2045 blocked binding and internalization of the Tc-99m-P2045, indicating the specificity of the technique. Up to four 96-well plates could be imaged simultaneously, thereby demonstrating the high capacity of the system. This novel in vitro approach provides a new method to test enhanced gene transfer as new vectors are developed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:51:30