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Titolo:
Continuous stimulation by normal luminal bacteria is essential for the development and perpetuation of colitis in Tg is an element of 26 mice
Autore:
Veltkamp, C; Tonkonogy, SL; De Jong, YP; Albright, C; Grenther, WB; Balish, E; Terhorst, C; Sartor, RB;
Indirizzi:
Univ N Carolina, Sch Med, Dept Med, Div Digest Dis & Nutr,Ctr GI Biol & Dis, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 & Dis, Chapel Hill, NC 27599 USA N Carolina State Univ, Coll Vet Med, Raleigh, NC USA N Carolina State Univ Raleigh NC USA Univ, Coll Vet Med, Raleigh, NC USA Beth Israel Hosp, Div Immunol, Boston, MA USA Beth Israel Hosp Boston MA USA Israel Hosp, Div Immunol, Boston, MA USA Univ Wisconsin, Dept Surg, Madison, WI USA Univ Wisconsin Madison WI USAUniv Wisconsin, Dept Surg, Madison, WI USA Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI USA Univ Wisconsin Madison WI USA t Med Microbiol & Immunol, Madison, WI USA
Titolo Testata:
GASTROENTEROLOGY
fascicolo: 4, volume: 120, anno: 2001,
pagine: 900 - 913
SICI:
0016-5085(200103)120:4<900:CSBNLB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY BOWEL-DISEASE; CD4(+) T-CELLS; CHRONIC INTESTINAL INFLAMMATION; IMMUNE-SYSTEM ACTIVATION; INTERLEUKIN-10-DEFICIENT MICE; MUCOSAL INFLAMMATION; TRANSGENIC RATS; DEFICIENT MICE; SCID MICE; B-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sartor, RB Univ N Carolina, Sch Med, Dept Med, Div Digest Dis & Nutr,Ctr GI Biol & Dis, CB 7038,Room 032A Glaxo Bldg, Chapel Hill, NC 27599 USA Univ N Carolina CB 7038,Room 032A Glaxo Bldg Chapel Hill NC USA 27599
Citazione:
C. Veltkamp et al., "Continuous stimulation by normal luminal bacteria is essential for the development and perpetuation of colitis in Tg is an element of 26 mice", GASTROENTY, 120(4), 2001, pp. 900-913

Abstract

Background & Aims: Normal resident bacteria are required for development of colitis in several rodent models. We determined whether bacterial stimulation is necessary for both induction and perpetuation of mucosal inflammation and T-cell activation in Tg epsilon 26 mice, in which transplantation ofwild-type bone marrow (BM double right arrow Tg epsilon 26) causes colitisunder specific pathogen-free (SPF) conditions. Methods: BM from (C57BL/6 XCBA/J) Fl mice was transplanted into germfree (GF) or SPF Tg epsilon 26 mice. Mesenteric lymph node (MLN) cells from these mice were then transferredinto SPF or GF recipients. Colitis and activation of MLN cells were measured by histologic scores, membrane marker analysis, and intracellular cytokine staining. Cytokine secretion by MLN cells stimulated by anti-CD3 or by luminal or epithelial antigens was measured by ELISA. Results: Colitis did not develop when BM was transferred into GF recipient mice (BM double right arrow GF Tg epsilon 26). T lymphocytes that secreted interferon gamma upon activation were present in the MLN of BM double right arrow GF Tg epsilon 26 mice, albeit in lower frequency than in control BM double right arrow SPFTg epsilon 26 mice, Furthermore, transfer of MLN cells from BM double right arrow SPF Tg epsilon 26 mice into SPF Tg epsilon 26 recipients induced active colitis, but not if the same cells were transferred into GF Tg epsilon26 recipients. Although CD4 T cells were detected in the colonic mucosa ofGF recipients, no inflammation was observed for at least 31 weeks. In a reciprocal experiment, MLN cells from BM double right arrow GF Tg epsilon 26 mice without colitis transferred disease to SPF Tge26 recipients within 2-4weeks. Conclusions: Activated T cells are present in the mucosa of BM double right arrow GF Tg epsilon 26 mice but are incapable of inducing disease unless colonic bacteria are present. Moreover, pathogenic T cells require the continuous presence of colonic bacteria to sustain colitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 02:44:21