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Titolo:
Development of a sensitive enzyme immunoassay for the detection of phenyl-beta-D-thioglucuronide in human urine
Autore:
Staimer, N; Gee, SJ; Hammock, BD;
Indirizzi:
Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 is, Dept Entomol, Davis, CA 95616 USA Univ Calif Davis, Canc Res Ctr, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 is, Canc Res Ctr, Davis, CA 95616 USA
Titolo Testata:
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY
fascicolo: 3-4, volume: 369, anno: 2001,
pagine: 273 - 279
SICI:
0937-0633(200102)369:3-4<273:DOASEI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; TANDEM MASS-SPECTROMETRY; GLUCURONIDATION; NAPHTHALENE; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Staimer, N Univ Calif Davis, Dept Entomol, 1 Shields Ave, Davis, CA 95616 USA Univ Calif Davis 1 Shields Ave Davis CA USA 95616 CA 95616 USA
Citazione:
N. Staimer et al., "Development of a sensitive enzyme immunoassay for the detection of phenyl-beta-D-thioglucuronide in human urine", FRESEN J AN, 369(3-4), 2001, pp. 273-279

Abstract

Immunoassays for the measurement of glucuronides in human urine can be a helpful tool for the assessment of human exposure to toxic chemicals. Therefore an enzyme immunoassay (EIA) for the specific detection of phenyl-beta -D-thioglucuronide was developed. The immunoconjugate was formed by couplingp-amino-phenyl-beta -D-thioglucuronide to the carrier protein thyroglobulin leaving an exposed glucuronic acid. The hapten-protein conjugate was adsorbed to gold colloids in order to enhance the immunogenic effect. Rabbits were injected with the immunogold conjugates to raise polyclonal antibodies. The resulting competitive assay showed an inhibition by phenyl-beta -D-thioglucuronide at sample concentrations of 23.0 +/- 1.3 ng/mL (50% B/B-o) anda high cross-reactivity to p-aminophenyl-beta -D-thioglucuronide (120%). Little cross-reactivities (< 2%) were observed for potential urinary cross reactants. In addition human urine samples were incubated with <beta>-glucuronidase in order to investigate the EIA for specific matrix effects. An integration of high-performance liquid chromatography (HPLC) and EIA was developed in an attempt to decrease the matrix effects and increase the sensitivity of the overall method. The hyphenated technique HPLC-EIA map be used tomonitor human exposure to toxic thiophenol which is excreted by mammals asurinary phenyl thioglucuronide.

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Documento generato il 27/09/20 alle ore 12:23:38