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Titolo:
POLYMERASE CHAIN REACTION-BASED DETECTION OF TROPHERYMA-WHIPPELII IN CENTRAL-NERVOUS-SYSTEM WHIPPLES-DISEASE
Autore:
LYNCH T; ODEL J; FREDERICKS DN; LOUIS ED; FORMAN S; ROTTERDAM H; FAHN S; RELMAN DA;
Indirizzi:
COLUMBIA UNIV COLL PHYS & SURG,DEPT NEUROL,630 W 168TH ST,ROOM BB-307NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,DEPT OPHTHALMOL NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,DEPT PATHOL NEW YORK NY 10032 COLUMBIA UNIV COLL PHYS & SURG,GERTRUDE H SERGIEVSKY CTR NEW YORK NY 10032 NEW YORK MED COLL,DEPT OPHTHALMOL,WESTCHESTER CTY MED CTR NEW YORK NY00000 STANFORD UNIV,DEPT MED STANFORD CA 94305 STANFORD UNIV,DEPT IMMUNOL & MICROBIOL STANFORD CA 94305 VET AFFAIRS PALO ALTO HLTH CARE SYST PALO ALTO CA 00000
Titolo Testata:
Annals of neurology
fascicolo: 1, volume: 42, anno: 1997,
pagine: 120 - 124
SICI:
0364-5134(1997)42:1<120:PCRDOT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-BLOOD; DIAGNOSIS; BACILLUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
T. Lynch et al., "POLYMERASE CHAIN REACTION-BASED DETECTION OF TROPHERYMA-WHIPPELII IN CENTRAL-NERVOUS-SYSTEM WHIPPLES-DISEASE", Annals of neurology, 42(1), 1997, pp. 120-124

Abstract

Whipple's disease of the central nervous system (CNS) may be associated with normal intestinal histology as a result of minimal or patchy involvement. The diagnosis is difficult and is frequently made post mortem. We studied 6 patients with clinically suspected CNS Whipple's disease; 2 had oculomasticatory myorhythmia (OMM) fitting criteria for a diagnosis of definite CNS Whipple's disease. One of the 2 had duodenalhistology highly suggestive of Whipple's disease; the other 5 patients had normal duodenal histology DNA was extracted from paraffin-embedded duodenal tissues in all patients and frozen pontine tissue in 1. Two primer pairs (W3F-W4R, W3F-W2R) were used in separate polymerase chain reactions (PCRs) to amplify fragments of Tropheryma whippelii 16S rDNA from these tissue samples. PCR amplicons were detected only in theduodenal tissues from the 2 patients with OMM. The sequences of theseamplicons were identical to the corresponding region of the previously published Tropheryma whippelii 16S rDNA sequence. PCR-based assays of intestinal or brain tissue may be of value for confirming, and possibly refuting, a clinical diagnosis of CNS Whipple's disease in a patient with any combination of dementia, supranuclear gaze palsy, hypothalamic manifestations, myoclonus, seizures, ataxia, or OMM, especially when tissue histology is unrevealing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:14:15