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Titolo:
Comparison of hydrolytic activity in water and heptane for thirty-two commercial lipase preparations
Autore:
Pencreach, G; Baratti, JC;
Indirizzi:
Univ Mediterranee, Fac Sci Luminy Biocatalyse & Chim Fine, CNRS UMR 6111, Marseille, France Univ Mediterranee Marseille France ne, CNRS UMR 6111, Marseille, France
Titolo Testata:
ENZYME AND MICROBIAL TECHNOLOGY
fascicolo: 4-5, volume: 28, anno: 2001,
pagine: 473 - 479
SICI:
0141-0229(20010308)28:4-5<473:COHAIW>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-CEPACIA LIPASE; ORGANIC-SOLVENTS; CATALYTIC ACTIVITY; MEDIA; IMMOBILIZATION; DEPENDENCE; STABILITY; SUPPORTS;
Keywords:
lipase; organic media; aqueous media; p-nitrophenyl palmitate hydrolysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Baratti, JC Univ Mediterranee, Fac Sci Luminy Biocatalyse & Chim Fine, CNRS UMR 6111, Marseille, France Univ Mediterranee Marseille France 6111, Marseille, France
Citazione:
G. Pencreac'h e J.C. Baratti, "Comparison of hydrolytic activity in water and heptane for thirty-two commercial lipase preparations", ENZYME MICR, 28(4-5), 2001, pp. 473-479

Abstract

The protein content and the rates of hydrolysis of p-nitrophenyl palmitate(pNPP) in water (soluble enzyme and emulsified substrate) and in heptane (soluble substrate and insoluble enzyme) were measured for thirty-two commercial lipase preparations. The protein content of the powders varied in a wide range as well as the activity on emulsified pNPP showing the high heterogeneity of the commercial samples. Activity in heptane also varied but to alesser extent than that in water. There was no direct correlation between activities in water and in heptane as assayed with the same hydrolytic reaction. The ratio of activity in heptane to that in water, R-O/A ratio, was introduced to characterize activity in organic media. Six lipases showed R-O/A values higher than 1 demonstrating a higher activity in organic solvent than in water. A linear correlation of R-O/A with activity in water (log plot) suggested the strong influence of diffusional limitations on activity of solid enzyme suspended in organic solvents. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:50:34