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Titolo:
Influence of different carbon fibers on salicylate microbiosensor performance
Autore:
De Carvalho, RM; Neto, GD; Kubota, LT;
Indirizzi:
UNICAMP, Inst Chem, BR-13083970 Campinas, SP, Brazil UNICAMP Campinas SP Brazil BR-13083970 BCBR-13083970 Campinas, SP, Brazil Univ Sao Francisco, Fac Pharmaceut Sci, BR-12900000 Braganca Paulista, SP,Brazil Univ Sao Francisco Braganca Paulista SP Brazil BR-12900000 BCa, SP,Brazil
Titolo Testata:
ELECTROANALYSIS
fascicolo: 2, volume: 13, anno: 2001,
pagine: 131 - 136
SICI:
1040-0397(200102)13:2<131:IODCFO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICRODISK ARRAY ELECTRODE; IN-VIVO; ELECTROCHEMICAL DETECTION; AMPEROMETRIC BIOSENSOR; CHROMAFFIN CELLS; SAMPLE VOLUMES; DYNAMICS; DOPAMINE; ELECTROPHORESIS; EXOCYTOSIS;
Keywords:
carbon fiber electrode; salicylate microbiosensor; chemical amplification and electroanalysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kubota, LT UNICAMP, Inst Chem, POB 6154, BR-13083970 Campinas, SP, Brazil UNICAMP POB 6154 Campinas SP Brazil BR-13083970 BCs, SP, Brazil
Citazione:
R.M. De Carvalho et al., "Influence of different carbon fibers on salicylate microbiosensor performance", ELECTROANAL, 13(2), 2001, pp. 131-136

Abstract

Amperometric salicylate microbiosensors were constructed by salicylate hydroxylase (SH) immobilization on different carbon fibers electrodes. The characteristics of the microbiosensor prepared with different carbon fibers were investigated. Five different carbon fibers were investigated. Toray M40 was very unstable, although reasonable performance compared with others wasobserved. The carbon fiber Toray-T800 was found to be the best amperometric microbiosensor for salicylate, presenting two linear response ranges between 0.1-1.93 mu mol L-1 (sensitivity of 56.0 +/- 0.4 mA mol(-1) L) and 1.96-12.0 mu mol L-1 (sensitivity of 20.8 +/- 0.4 mA mol(-1) L), with a detection limit of 6.4 +/- 0.9 x 10(-8) m L-1 and response time of 3.5 seconds. The relative standard deviation for 10 determinations was 4.1%. The high sensitivity of the microbiosensor was assigned to the chemical amplification process. Salicylate determination can be carried out with applied potentials higher than 150 mV (vs. SCE), with good precision. The useful lifetime of the biosensor was 100 continuous determinations without significant change.

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Documento generato il 26/01/21 alle ore 04:42:42