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Titolo:
EXPRESSION OF POKEWEED ANTIVIRAL PROTEIN IN TRANSGENIC PLANTS INDUCESVIRUS-RESISTANCE IN GRAFTED WILD-TYPE PLANTS INDEPENDENTLY OF SALICYLIC-ACID ACCUMULATION AND PATHOGENESIS-RELATED PROTEIN-SYNTHESIS
Autore:
SMIRNOV S; SHULAEV V; TUMER NE;
Indirizzi:
RUTGERS STATE UNIV,CTR AGR MOL BIOL,POB 231 NEW BRUNSWICK NJ 08903 RUTGERS STATE UNIV,CTR AGR MOL BIOL NEW BRUNSWICK NJ 08903 RUTGERS STATE UNIV,DEPT PLANT PATHOL NEW BRUNSWICK NJ 08903
Titolo Testata:
Plant physiology
fascicolo: 3, volume: 114, anno: 1997,
pagine: 1113 - 1121
SICI:
0032-0889(1997)114:3<1113:EOPAPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC ACQUIRED-RESISTANCE; RIBOSOME-INACTIVATING PROTEINS; SP STRAIN WCS417R; PHYTOLACCA-AMERICANA; FUSARIUM-WILT; PSEUDOMONAS-FLUORESCENS; TRANSLOCATED SIGNAL; MOSAIC-VIRUS; TOBACCO; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Smirnov et al., "EXPRESSION OF POKEWEED ANTIVIRAL PROTEIN IN TRANSGENIC PLANTS INDUCESVIRUS-RESISTANCE IN GRAFTED WILD-TYPE PLANTS INDEPENDENTLY OF SALICYLIC-ACID ACCUMULATION AND PATHOGENESIS-RELATED PROTEIN-SYNTHESIS", Plant physiology, 114(3), 1997, pp. 1113-1121

Abstract

Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic tobacco (Nicotiana tabacum) plants expressing PAP or a variant (PAP-v) were shown to be resistant to a broad spectrum of plant viruses. Expression of PAP-v in transgenic plants inducessynthesis of pathogenesis-related proteins and a very weak (<2-fold) increase in salicylic acid levels. Using reciprocal grafting experiments, we demonstrate here that transgenic tobacco rootstocks expressing PAP-v induce resistance to tobacco mosaic virus infection in both N. tabacum NN and nn scions. Increased resistance to potato virus X was also observed in N. tabacum nn scions grafted on transgenic rootstocks. PAP expression was not detected in the wild-type scions or rootstocks that showed virus resistance, nor was there any increase in salicylic acid levels or pathogenesis-related protein synthesis. Crafting experiments with transgenic plants expressing an inactive PAP mutant demonstrated that an intact active site of PAP is necessary for induction of virus resistance in wild-type scions. These results indicate that enzymatic activity of PAP is responsible for generating a signal that renders wild-type scions resistant to virus infection in the absence of increased salicylic acid levels and pathogenesis-related protein synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:56:51