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Titolo:
Characterization of equine microsatellites and microsatellite-linked repetitive elements (eMLREs) by efficient cloning and genotyping methods
Autore:
Tozaki, T; Mashima, S; Hirota, K; Miura, N; Miura, NHC; Tomita, M;
Indirizzi:
Lab Racing Chem, Dept Mol Genet, Utsunomiya, Tochigi 3200851, Japan Lab Racing Chem Utsunomiya Tochigi Japan 3200851 , Tochigi 3200851, Japan Showa Univ, Sch Pharmaceut Sci, Dept Physiol Chem, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 , Dept Physiol Chem, Tokyo 1428555, Japan
Titolo Testata:
DNA RESEARCH
fascicolo: 1, volume: 8, anno: 2001,
pagine: 33 - 45
SICI:
1340-2838(20010228)8:1<33:COEMAM>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; DINUCLEOTIDE REPEAT; HORSE GENOME; EQUUS-CABALLUS; LINKAGE MAP; LOCI; MARKERS; DNA; ASSIGNMENT; GENE;
Keywords:
equine microsatellite; repetitive element; polymorphism; MLRE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Tozaki, T Lab Racing Chem, Dept Mol Genet, 1731-2 Tsuruta Cho, Utsunomiya,Tochigi 3200851, Japan Lab Racing Chem 1731-2 Tsuruta Cho Utsunomiya Tochigi Japan 3200851
Citazione:
T. Tozaki et al., "Characterization of equine microsatellites and microsatellite-linked repetitive elements (eMLREs) by efficient cloning and genotyping methods", DNA RES, 8(1), 2001, pp. 33-45

Abstract

We performed efficient cloning and genotyping methods for isolation of a large number of polymorphic microsatellites. The methods contain the time-efficient cloning method of constructing microsatellite-enriched libraries and the economic genotyping method of fluorescent labeling of PCR products. Eighty novel equine microsatellites cloned were efficiently isolated from the enrichment library and analyzed for genotype polymorphism. Of these, 72 microsatellites were analyzed with a good resolution. The average heterozygosity of all loci was 0.52, and the number of alleles ranged from one to 9 with an average of 4.5 alleles. The other eight loci showed multiple bands of PCR products, suggesting the occurrence of microsatellites in a repetitive element, in which the number of microsatellite repeats varies among different members of the repetitive element. We found five homologous groups at flanking regions in comparison with theflanking regions of microsatellites from DNA databases. One of them showedhomology to equine repetitive element-2. In the other four homologous groups, the two groups were named equine microsatellite-linked repetitive element-1 (eMLRE-1) and equine microsatellite-linked repetitive element-2 (eMLRE-2) as novel equine repetitive elements identified from equine genome. These data should help the analysis of equine DNA sequences and the design of equine genome markers.

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Documento generato il 13/07/20 alle ore 16:42:08