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Titolo:
Application of laser scanning cytometry for evaluation of DNA ploidy in routine cytologic specimens
Autore:
Wojcik, EM; Saraga, SA; Jin, JK; Hendricks, JB;
Indirizzi:
Loyola Univ, Med Ctr, Dept Pathol, Maywood, IL 60153 USA Loyola Univ Maywood IL USA 60153 Ctr, Dept Pathol, Maywood, IL 60153 USA
Titolo Testata:
DIAGNOSTIC CYTOPATHOLOGY
fascicolo: 3, volume: 24, anno: 2001,
pagine: 200 - 205
SICI:
8755-1039(200103)24:3<200:AOLSCF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSITIONAL-CELL-CARCINOMA; PARAFFIN-EMBEDDED TISSUE; URINARY-BLADDER; IMAGE CYTOMETRY; FLOW-CYTOMETRY; SOLID TUMORS; FOLLOW-UP; PATHOLOGY;
Keywords:
laser scanning cytometry; cytology; DNA ploidy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Wojcik, EM Loyola Univ, Med Ctr, Dept Pathol, 2160 S 1st Ave, Maywood, IL 60153 USA Loyola Univ 2160 S 1st Ave Maywood IL USA 60153 d, IL 60153 USA
Citazione:
E.M. Wojcik et al., "Application of laser scanning cytometry for evaluation of DNA ploidy in routine cytologic specimens", DIAGN CYTOP, 24(3), 2001, pp. 200-205

Abstract

The laser scanning cytometer (LSC) is a relatively new instrument that combines the features of both flow and static image cytometry. The purpose of this study was to examine the application of the LSC for evaluation of DNA ploidy in routine cytologic specimens. The material for this study consisted of 60 routine cytologic specimens obtained from 33 males and 27 females ranging in age from 23-87 yr (mean, 58 yr). The specimens were simultaneously stained with propidium iodide and FITC-cytokeratin. either on Thin-Prep slide (35 cases) ol in a concentrated cell suspension (25 cases). In each case a minimum of 500 cells was evaluated (range, 527-17,963; mean, 3,889). All abnormal cell populations were relocated for the presence of malignant cells. The results were defined as diploid and aneuploid/tetraploid. In 10 bladder washes, the results of LSC were compared to results of flow cytometry. Out of 60 specimens, 7 (11%: 6 bladder washes and I renal wash) were excluded doe to low cellularity. Of the remaining 53 cases, 11 (20%) were aneuploid/tetraploid and 42 (80%) were diploid. All but one cytologically diagnosed malignancy had abnormal DNA content. Additionally, two bladder washes diagnosed as suspicious and atypical were aneuploid All abnomal LSC resultswere confirmed by relocation of the cells. The concordance between pow cytometry and LSC in the 10 control bladder washes was 100%. In conclusion, LSC proved to be a suitable instrument for the evaluation of DNA ploidy in routine cytologic specimens. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 17:58:36