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Titolo:
Detection of malignant effusions: Comparison of a telomerase assay and cytologic examination
Autore:
Braunschweig, R; Yan, P; Guilleret, I; Delacretaz, F; Bosman, FT; Mihaescu, A; Benhattar, J;
Indirizzi:
CHU Vaudois, Inst Pathol, CH-1011 Lausanne, Switzerland CHU Vaudois Lausanne Switzerland CH-1011 , CH-1011 Lausanne, Switzerland
Titolo Testata:
DIAGNOSTIC CYTOPATHOLOGY
fascicolo: 3, volume: 24, anno: 2001,
pagine: 174 - 180
SICI:
8755-1039(200103)24:3<174:DOMECO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; DIAGNOSTIC-VALUE; PULMONARY TUBERCULOSIS; PLEURAL EFFUSIONS; SEROUS EFFUSIONS; FLOW-CYTOMETRY; IMAGE-ANALYSIS; LUNG-CANCER; TUMOR; IMMUNOCYTOCHEMISTRY;
Keywords:
telomerase; TRAP assay; malignant effusion; cytology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Braunschweig, R CHU Vaudois, Inst Pathol, Bugnon 25, CH-1011 Lausanne, Switzerland CHU Vaudois Bugnon 25 Lausanne Switzerland CH-1011 erland
Citazione:
R. Braunschweig et al., "Detection of malignant effusions: Comparison of a telomerase assay and cytologic examination", DIAGN CYTOP, 24(3), 2001, pp. 174-180

Abstract

Telomerase is inactive in most somatic cells, but has been found to be reactivated in a majority of cancers. Our principal goal was to rest whether the presence of telomerase activity concurred with positive cytology, and was thus of potential use in detecting cancer cells in effusions. The telomeric repeat amplification protocol (TRAP) assay and cytological examination were performed in a blinded fashion on 91 unselected effusions, for which laboratory processing was done according to standard procedures. In our series, 30% (27/91) of samples were found to be malignant by cytology. Of these,19 (70%) were also positive in the TRAP assay. Of the 8 telomerase-negative cytology-positive samples, RNA integrity was generally poor, indicating suboptimal sample conservation for molecular analysis. Negative cytology in the presence of telomerase activity was observed in 17 effusions. Of these,11 were from patients with advanced cancer, and thus a diagnosis of malignant effusion should be suspected. The TRAP assay for telomerase activity holds promise in the analysis of effusions, but its routine use as an adjunctto cytology awaits further confirmation of its positive predictive value. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 22:57:14