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Titolo:
Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata
Autore:
Gagne, F; Blaise, C; Salazar, M; Salazar, S; Hansen, PD;
Indirizzi:
St Lawrence Ctr, Montreal, PQ H27 2E7, Canada St Lawrence Ctr Montreal PQCanada H27 2E7 , Montreal, PQ H27 2E7, Canada Appl Biomonitoring, Seattle, WA USA Appl Biomonitoring Seattle WA USAAppl Biomonitoring, Seattle, WA USA Tech Univ Berlin, Berlin, Germany Tech Univ Berlin Berlin GermanyTech Univ Berlin, Berlin, Germany
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
fascicolo: 2, volume: 128, anno: 2001,
pagine: 213 - 225
SICI:
1532-0456(200102)128:2<213:EOEEOM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAINBOW-TROUT HEPATOCYTES; STIMULATED XENOPUS-LAEVIS; OYSTER CRASSOSTREA-GIGAS; PLASMA VITELLOGENIN; MYTILUS-EDULIS; IN-VITRO; SEWAGE; LIVER; OOGENESIS; ESTRADIOL-17-BETA;
Keywords:
freshwater mussels estrogen binding sites; vitellins; municipal effluents; lipids; sugars; growth;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Gagne, F St Lawrence Ctr, 105 McGill St,7th Floor, Montreal, PQ H27 2E7, Canada St Lawrence Ctr 105 McGill St,7th Floor Montreal PQ Canada H27 2E7
Citazione:
F. Gagne et al., "Evaluation of estrogenic effects of municipal effluents to the freshwater mussel Elliptio complanata", COMP BIOC C, 128(2), 2001, pp. 213-225

Abstract

Municipal effluents are an important source of estrogens to the aquatic environment. The purpose of this study was to examine the estrogenicity of municipal effluents to the indigenous freshwater mussel, Elliptio complanata. First, estradiol-binding sites in gonad homogenates were characterized to determine the binding affinity and specificity of estrogens. Mussels were exposed to increasing concentrations of a municipal effluent for 96 h at 15 degreesC. In another experiment, mussels were placed in cages and submergedfor 62 days at 1.5 km upstream and 5 km downstream of a municipal effluentplume in the St. Lawrence River. Mussels were harvested for assessment of vitellogenin-like proteins in the hemolymph and determination of total lipid, carbohydrate and protein in the gonad. The presence of specific estrogen-binding sites was found in both male and female gonads. Binding of estradiol to cytosol proteins reached saturation, yielding a dissociation constantof 0.4 nM. Vitellogenin (Vg) levels increased significantly in both the hemolymph and the gonad after exposure to the effluent. Moreover, females appeared to be more sensitive than males to producing Vg. Mussels exposed in situ to contaminated surface waters had higher levels of Vg at the downstream site, again, females had higher levels of Vg than did males. On the otherhand, lipid and sugar levels in male gonads were significantly increased at the downstream site. Moreover, mussels at the downstream site had decreased shell growth length and increased total and soft tissue weights. We conclude that municipal effluents contain bio-available xenoestrogens at levelssufficient to elicit effects in freshwater mussels. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 00:29:42