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Titolo:
In vitro modulation of human lung cancer cell line invasiveness by antisense cDNA of tissue factor pathway inhibitor-2
Autore:
Lakka, SS; Konduri, SD; Mohanam, S; Nicolson, GL; Rao, JS;
Indirizzi:
Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Inst Mol Med, Huntington Beach, CA USA Inst Mol Med Huntington Beach CA USA t Mol Med, Huntington Beach, CA USA
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 3, volume: 18, anno: 2000,
pagine: 239 - 244
SICI:
0262-0898(2000)18:3<239:IVMOHL>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR; UROKINASE RECEPTOR; MATRIX DEGRADATION; GROWTH-FACTOR; FACTOR VIIA; EXPRESSION; INVASION; FIBROBLASTS; CLONING; IDENTIFICATION;
Keywords:
TFPI-2; lung cancer; invasion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, POB 1649, Peoria, IL 61656 USA Univ Illinois POB 1649 Peoria IL USA 61656 9, Peoria, IL 61656 USA
Citazione:
S.S. Lakka et al., "In vitro modulation of human lung cancer cell line invasiveness by antisense cDNA of tissue factor pathway inhibitor-2", CLIN EXP M, 18(3), 2000, pp. 239-244

Abstract

Human tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type serine protease inhibitor that inhibits plasmin, trypsin, chymotrypsin, cathepsin Gand plasma kallikrein but not urokinase (uPA) or tissue-type plasminogen activator and thrombin. Earlier studies from our and other laboratories haveshown that the production of TFPI-2 is downregulated during the progression of various cancers. To investigate the role of TFPI-2 in the invasion andmetastasis of lung tumors, the human lung cancer cell line A549, which produces high levels of TFPI-2, was stably transfected with a vector capable of expressing an antisense transcript complementary to the full-length TFPI-2 mRNA. Northern blot analysis was used to quantify the TFPI-2 mRNA transcript, and western blot analysis was used to measure TFPI-2 protein levels inparental cells and stably transfected (vector and antisense) clones. The levels of TFPI-2 mRNA and protein were significantly less in antisense clones than in the parental and vector controls. The invasive potential of the parental cells and stably transfected vector clones in vitro, as measured bythe Matrigel invasion assay, was also markedly less than that of antisenseclones. Further characterization of these clones showed that more cells migrated from antisense clones than from parental and vector clones. These data suggest that TFPI-2 is critical for the invasion and metastasis of lung cancer and that the downregulation of TFPI-2 production may be a feasible approach to increase invasiveness and metastasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 13:27:38