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Titolo:
Small molecule-based laser inactivation of inositol 1,4,5-trisphosphate receptor
Autore:
Inoue, T; Kikuchi, K; Hirose, K; Iino, M; Nagano, T;
Indirizzi:
Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 ceut Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Grad Sch Med, Dept Pharmacol, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 harmacol, Bunkyo Ku, Tokyo 1130033, Japan Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130033, Japan Japan Sci& Technol Corp Tokyo Japan 1130033 yo Ku, Tokyo 1130033, Japan
Titolo Testata:
CHEMISTRY & BIOLOGY
fascicolo: 1, volume: 8, anno: 2001,
pagine: 9 - 15
SICI:
1074-5521(200101)8:1<9:SMLIOI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRISPHOSPHATE RECEPTOR; INTRACELLULAR CA-2+; PROTEIN FUNCTION; SMOOTH-MUSCLE; CALCIUM; CELLS; STORES; CA2+; IP3;
Keywords:
calcium release; inositol 1,4,5-trisphosphate receptor; laser inactivation; small-molecular probe;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Nagano, T Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1130033 Tokyo 1130033, Japan
Citazione:
T. Inoue et al., "Small molecule-based laser inactivation of inositol 1,4,5-trisphosphate receptor", CHEM BIOL, 8(1), 2001, pp. 9-15

Abstract

Background: Chromophore-assisted laser inactivation (CALI) is a powerful method for the study of in situ protein function in cellular processes. By using CALI, it is possible to abrogate the function of a target protein withunprecedented spatial and temporal resolution. However. CALI has some limitations, which restrict wider biological application, owing mainly to the use of antibody for target recognition. To circumvent the limitations, we have developed small molecule-based CALI (smCALI). Results: The inositol 1,4.5-trisphosyhate receptor (IP3R) was selected as the target protein and a malachite green-conjugated IF? analog. MGIP(3), was used as a small-molecular probe. We examined the effect of MGIP(3)-based CALI on Ca2+ Irelease via IP3R using permeabilized smooth muscle cells. When the cells were treated with MGIP3 Followed by laser irradiation, the IP3-induced Ca2+ release rate was decreased in a concentration- and irradiationtime-dependenr manner. The effect was specific for IP3R. because the Ca2+ uptake function of the co-localized sarco/endoplasmic reticulum Ca2+-ATPasewas not affected was not affected. Conclusions: IP3R was specifically inactivated by smCALI using MGIP(3). The efficiency of inactivation was calculated to be substantially greater than that of antibody-based CALI. The efficient and specific inactivation of IP3R would allow us to obtain an insight into spatiotemporal roles of IP3R in various cell functions. Our results may be considered to be a first step for a wider application of smCALI as a useful method to study spatiotemporal protein functions. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 05:17:20