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Titolo:
Cytological and lectin histochemical characterization of secretion production and secretion composition in the tubular glands of the canine anal sacs
Autore:
Meyer, W; Tsukise, A; Neurand, K; Hirabayashi, Y;
Indirizzi:
Sch Vet Med, Inst Anat, Hannover, Germany Sch Vet Med Hannover GermanySch Vet Med, Inst Anat, Hannover, Germany Nihon Univ, Coll Bioresource Sci, Dept Vet Anat, Fujisawa, Kanagawa, JapanNihon Univ Fujisawa Kanagawa Japan t Vet Anat, Fujisawa, Kanagawa, Japan Nagoya City Univ, Dept Anat 2, Nagoya, Aichi, Japan Nagoya City Univ Nagoya Aichi Japan v, Dept Anat 2, Nagoya, Aichi, Japan
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 3, volume: 168, anno: 2001,
pagine: 203 - 219
SICI:
1422-6405(2001)168:3<203:CALHCO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCATION END-PRODUCTS; ACRIDINE-ORANGE; LYSOSOMAL PH; LIPOFUSCIN; APOCRINE; PROTEIN; GLYCOSYLATION; LOCALIZATION; DISEASE; TISSUE;
Keywords:
cytology; lectin histochemistry; secretion production; secretion composition; tubular glands; anal sacs; dog;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, W Tierarztlichen Hsch Hannover, Inst Anat, Bischofsholer Damm 15, D-30173 Hannover, Germany Tierarztlichen Hsch Hannover Bischofsholer Damm 15Hannover Germany D-30173
Citazione:
W. Meyer et al., "Cytological and lectin histochemical characterization of secretion production and secretion composition in the tubular glands of the canine anal sacs", CELLS T ORG, 168(3), 2001, pp. 203-219

Abstract

The study reports on secretion production and composition in the tubular glands of the canine anal sacs. For this purpose, light and electron microscopical (TEM, SEM) as well as several histochemical methods for the demonstration of lysosomal acidity, lipofuscin, and complex carbohydrates were used. The glandular tubules exhibited a pseudostratified epithelium with secretory cells of a different shape as related to secretion production activity,and regionally varying amounts of basal cells. Flat, cuboidal or columnar cells with or without apocrine-like protrusions were assembled in one glandular endpiece, although grouping of these cell types often occurred. Activesecretory cells were columnar with many cytoplasmic vesicles and a typically merocrine and/or micro-apocrine exocytosis of vesicle contents. Additionally, many lysosomes of different sizes could be found, whereby in aged cells giant secondary lysosomes (auto-phagolysosomes, about 7 mum in diameter)occupied the major cell part. These giant lysosomes were shed by an apocrine-like process forming a final bottleneck stage of the upper cell part, and consisted of ceroid-type lipofuscin. The general carbohydrate histochemical and the lectin histochemical methods revealed that the secretion produced was composed of strongly concentrated neutral glycoproteins with the following saccharide residues: alpha -D-mannose, beta -D-galactose, beta -N-acetyl-D-glucosamine, alpha -L-fucose and N-acetyl-neuraminic acid (sialic acid); the luminal secretion contained only beta -D-galactose and, especially,N-acetyl-neuraminic acid. This luminal secretion showed a spatially orientated maturation beginning in terminal tubular regions and finishing near the excretory duct, independent of the different secretory cell types. The results obtained demonstrated highly active secretion production, with a regional variation in the glandular tubule, and at least three different modes of secretion by the secretory cells, whereby the shedding of giant lipofuscin granules seems to be very specific. The high amounts of sialic acids in the glycoproteins found may influence the rheological properties of the secretion by their water-binding capacities. Copyright (C) 2001 S. Kaiger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:40:14