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Titolo:
Synergistic effect of titanium alloy and collagen type I on cell adhesion,proliferation and differentiation of osteoblast-like cells
Autore:
Roehlecke, C; Witt, M; Kasper, M; Schulze, E; Wolf, C; Hofer, A; Funk, RHW;
Indirizzi:
Tech Univ Dresden, Dept Anat, D-01307 Dresden, Germany Tech Univ Dresden Dresden Germany D-01307 Anat, D-01307 Dresden, Germany Tech Univ Dresden, Dept Mat Sci, D-01307 Dresden, Germany Tech Univ Dresden Dresden Germany D-01307 Sci, D-01307 Dresden, Germany Tech Univ Dresden, Clin Reconstruct Surg, D-01307 Dresden, Germany Tech Univ Dresden Dresden Germany D-01307 Surg, D-01307 Dresden, Germany
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 3, volume: 168, anno: 2001,
pagine: 178 - 187
SICI:
1422-6405(2001)168:3<178:SEOTAA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORTHOPEDIC IMPLANT MATERIALS; HUMAN GINGIVAL FIBROBLASTS; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; INTRACELLULAR OSTEOPONTIN; PROGRESSIVE DEVELOPMENT; INTEGRIN EXPRESSION; FOCAL ADHESIONS; GENE-EXPRESSION; FLOW-CYTOMETRY;
Keywords:
titanium; osteoblasts; collagen type I; focal adhesions; immunocytochemistry; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Witt, M Tech Univ Dresden, Dept Anat, Fetscherstr 74, D-01307 Dresden, Germany Tech Univ Dresden Fetscherstr 74 Dresden Germany D-01307 Germany
Citazione:
C. Roehlecke et al., "Synergistic effect of titanium alloy and collagen type I on cell adhesion,proliferation and differentiation of osteoblast-like cells", CELLS T ORG, 168(3), 2001, pp. 178-187

Abstract

A number of studies have demonstrated the pivotal role of collagen in modulating cell growth and differentiation. In bone, where the extracellular matrix is composed of approximately 85% type I collagen, cellular interactionwith matrix components has been shown to be important in the regulation ofthe osteoblast phenotype. Preservation or enhancement of normal osteoblastfunction and appositional bone formation after implant placement represents a strategy that can be useful I for the purpose of improving osseointegration. In order to further improve biocompatibility, we combined two known favorable compounds, namely the titanium alloy, Ti6A14V, with type I collagen. We assessed the in vitro behavior of primary osteoblasts grown on both fibrillar collagen-coated and tropocollagen-coated Ti6A14V in comparison with uncoated titanium alloy, using an improved adsorption procedure. As parameters of biocompatibility, a variety of processes, including cell attachment, spreading, cytoskeletal organization, focal contact formation, proliferation and expression of a differentiated phenotype, were investigated. Our results demonstrated for the first time that in comparison to uncoated titanium alloy, collagen-coated alloy enhanced spreading and resulted in a more rapid formation of focal adhesions and their associated stress fibers. Growing on collagen-coated Ti6A14V, osteoblasts had a higher proliferative capacity and the intracellular expression of osteopontin was upregulated compared to uncoated titanium alloy. Type I collagen-coated titanium alloy exhibits favorable effects on the initial adhesion and growth activities of osteoblasts, which is encouraging for its potential use as bone graft material. Moreover, collagen type I may serve as an excellent biocompatible carrier for osteotropic factors such as cell adhesion molecules (e.g. fibronectin) or bone-specific growth factors. Copyright (C) 2001 S.Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 06:50:26