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Titolo:
Hepatocytes entrapped in alginate gel beads and cultured in bioreactor: Rapid repolarization and reconstitution of adhesion areas
Autore:
Falasca, L; Miccheli, A; Sartori, E; Tomassini, A; Devirgiliis, LC;
Indirizzi:
IRCCS L Spallanzani, Lab Microscopia Elettron, I-00149 Rome, Italy IRCCS LSpallanzani Rome Italy I-00149 pia Elettron, I-00149 Rome, Italy
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 3, volume: 168, anno: 2001,
pagine: 126 - 136
SICI:
1422-6405(2001)168:3<126:HEIAGB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HEPATOCYTES; EXTRACELLULAR-MATRIX; BIOARTIFICIAL LIVER; SANDWICH CONFIGURATION; NONPARENCHYMAL CELLS; MAINTENANCE; OXYGEN; GROWTH; RECONSTRUCTION; CYTOSKELETON;
Keywords:
hepatocytes; ultrastructure; cytoskeleton; alginate; bioreactor; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Falasca, L IRCCS L Spallanzani, Lab Microscopia Elettron, Via Portuense 292, I-00149 Rome, Italy IRCCS L Spallanzani Via Portuense 292 Rome Italy I-00149 Italy
Citazione:
L. Falasca et al., "Hepatocytes entrapped in alginate gel beads and cultured in bioreactor: Rapid repolarization and reconstitution of adhesion areas", CELLS T ORG, 168(3), 2001, pp. 126-136

Abstract

The maintenance of the differentiated hepatocyte phenotype and its specific physiological properties is known to depend on several factors, such as chemical signals, cell-cell and extracellular matrix molecular interactions,as well as the use of three-dimensional matrices. The entrapment of hepatocytes within Ca-alginate at high cell density and the culture under continuous flow favour the development of three-dimensional organization and promote expression of the differentiated hepatic phenotype. This system could represent an improvement in hepatocyte cultivation for basic studies of liverphysiology and metabolism; it could also be applicable in toxicology, hepatocyte transplantation or development of bioartificial organs. This report describes the effect of alginate entrapment and culture in a bioreactor on hepatocyte aggregate formation, with particular attention to the reestablishment of cell polarity, cell junctions and three-dimensional re-organization of the cytoskeleton. Oxygen supply and cell oxygen consumption rate were monitored in order to evaluate possible changes in hepatocyte energy requirement. Our data show that after only 6 h of perfusion in the bioreactor, actin and cytokeratin localize along the adhesion areas of the plasma membrane, in which reconstituted bile canaliculi were also observed. Moreover, thepresence of connexin at the level of joined membranes of neighbouring cells suggests the establishment of gap junctions between hepatocytes. After the first 30 min of perfusion the oxygen consumption rate remained constant throughout the experimental period. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 22:54:20