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Titolo:
Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects: applications to breast cancer
Autore:
Berger, F; Gambhir, SS;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
BREAST CANCER RESEARCH
fascicolo: 1, volume: 3, anno: 2001,
pagine: 28 - NIL_1
SICI:
1465-542X(2001)3:1<28:RAIIEO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON-EMISSION-TOMOGRAPHY; REPORTER GENE; IN-VIVO; CLINICAL-TRIAL; PET; THERAPY; ANIMALS; QUANTIFICATION; STRATEGIES; ONCOLOGY;
Keywords:
animal models; imaging; microPET; positron emission tomography; single photon emission computed tomography;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Gambhir, SS Univ Calif Los Angeles, Sch Med, Crump Inst Mol Imaging, Dept Mol & Med Pharmacol, Box 951770, Los Angeles, CA 90095 USA Univ Calif Los Angeles Box 951770 Los Angeles CA USA 90095 USA
Citazione:
F. Berger e S.S. Gambhir, "Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects: applications to breast cancer", BREAST CA R, 3(1), 2001, pp. 28-NIL_1

Abstract

A variety of imaging technologies is being investigated as tools for studying gene expression in living subjects. Two technologies that use radiolabeled isotopes are single photon emission computed tomography (SPECT) and positron emission tomography (PET). A relatively high sensitivity, a full quantitative tomographic capability, and the ability to extend small animal imaging assays directly into human applications characterize radionuclide approaches. Various radiolabeled probes (tracers) can be synthesized to target specific molecules present in breast cancer cells. These include antibodiesor ligands to target cell surface receptors, substrates for intracellular enzymes, antisense oligodeoxynucleotide probes for targeting mRNA, probes for targeting intracellular receptors, and probes for genes transferred intothe cell. We briefly discuss each of these imaging approaches and focus indetail on imaging reporter genes. In a PET reporter gene system for in vivo reporter gene imaging, the protein products of the reporter genes sequester positron emitting reporter probes. PET subsequently measures the PET reporter gene dependent sequestration of the PET reporter probe in living animals. We describe and review reporter gene approaches using the herpes simplex type 1 virus thymidine kinase and the dopamine type 2 receptor genes. Application of the reporter gene approach to animal models for breast cancer is discussed. Prospects for future applications of the transgene imaging technology in human gene therapy are also discussed. Both SPECT and PET provide unique opportunities to study animal models of breast cancer with directapplication to human imaging. Continued development of new technology, probes and assays should help in the better understanding of basic breast cancer biology and in the improved management of breast cancer patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:05:51