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Titolo:
New classification system for oxygenase components involved in ring-hydroxylating oxygenations
Autore:
Nam, JW; Nojiri, H; Yoshida, T; Habe, H; Yamane, H; Omori, T;
Indirizzi:
Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 2, volume: 65, anno: 2001,
pagine: 254 - 263
SICI:
0916-8451(200102)65:2<254:NCSFOC>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP STRAIN CA10; PSEUDOMONAS-PUTIDA; BIPHENYL DIOXYGENASE; 2-OXO-1,2-DIHYDROQUINOLINE 8-MONOOXYGENASE; NAPHTHALENE 1,2-DIOXYGENASE; CARBAZOLE 1,9A-DIOXYGENASE; DIHYDRODIOL DEHYDROGENASE; NUCLEOTIDE-SEQUENCES; TOLUENE DIOXYGENASE; GENETIC-ANALYSIS;
Keywords:
classification; ring-hydroxylating oxygenases; terminal oxygenase component; alpha-subunit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Omori, T Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Yayoi 1-1-1, Tokyo 1138657, Japan Univ Tokyo Yayoi 1-1-1 Tokyo Japan 1138657 Tokyo 1138657, Japan
Citazione:
J.W. Nam et al., "New classification system for oxygenase components involved in ring-hydroxylating oxygenations", BIOS BIOT B, 65(2), 2001, pp. 254-263

Abstract

Batie et al. [Chemistry and Biochemistry of Flavoenzymes, 3, 543-556 (1991)] proposed a classification system for ring-hydroxylating oxygenases in which the oxygenases are grouped into three classes in terms of the number ofconstituent components and the nature of the redox centers. But in recent gears, many ring-hydroxylating oxygenases have been newly identified and characterized, and found difficult to classify into these three classes. Typical examples are carbazole 1,9a-dioxygenase and 2-oxo-1,2-dihydroquinoline 8-monooxygenase, which have been classified into class Hi and class IB, respectively, from biochemical characteristics. However, a phylogenetic study showed that the terminal oxygenases of both are closely related to class IA. Because this discrepancy derived from counting all the components together, here we proposed a new scheme based on the homology of the amino acid sequences of the alpha subunits of the terminal oxygenase components. This new scheme strongly reflects the actual phylogenetic affiliation of the terminal oxygenase component. By comparing their sequences painwise using the CLUSTAL W program, 54 oxygenase components were classified into 4 groups (groups I, II, III, and IV). while group I contains broad-range oxygenases sharing low homology, groups II, Hi, and IV contain some typical oxygenases: benzoate/toluate dioxygenases for group II, naphthalene/polycyclic aromatic hydrocarbon dioxygenases for group Hi, and benzene/toluene/biphenyl dioxygenases for group IV. Our new scheme is simple and powerful, since an oxygenase component can be nearly automatically grouped when the DNA sequence Is available, and it fits very wed with the phylogenetic affiliation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 03:25:13