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Titolo:
DNA architecture and transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene
Autore:
Stojcevic, N; Moric, I; Begovic, J; Radoja, S; Konstantinovic, M;
Indirizzi:
Inst Mol Genet & Genet Engn, YU-11000 Belgrade, Yugoslavia Inst Mol Genet & Genet Engn Belgrade Yugoslavia YU-11000 ade, Yugoslavia
Titolo Testata:
BIOMOLECULAR ENGINEERING
fascicolo: 3, volume: 17, anno: 2001,
pagine: 113 - 117
SICI:
1389-0344(200103)17:3<113:DAATRO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTEGRATION HOST FACTOR; AMP RECEPTOR PROTEIN; CYCLIC-AMP; ACTIVATION; EXPRESSION; PROMOTERS; ACYLASE; CAMP; TANDEM; CLONING;
Keywords:
CRP; protein-DNA interaction; integration host factor; DNA bending; transcription regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Konstantinovic, M Inst Mol Genet & Genet Engn, Vojvode Stepe 444A,POB 446,YU-11000 Belgrade, Yugoslavia Inst Mol Genet & Genet Engn Vojvode Stepe 444A,POB 446 Belgrade Yugoslavia YU-11000
Citazione:
N. Stojcevic et al., "DNA architecture and transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene", BIOMOL ENG, 17(3), 2001, pp. 113-117

Abstract

The transcriptional regulation of Escherichia coli ATCC11105 penicillin amidase (pac) gene was studied by modifying DNA sequences responsible For promoter activation by cyclic AMP receptor protein (CRP). The nucleotide sequence of the 5'-flanking region of the pac gene contains putative tandem CRP binding sites positioned at -69/-70 and at -111/-112 with respect to the transcriptional start site. Our results obtained with either point mutations or insertion or deletion mutants (each of which rotated the helix structureat the CRP binding site one-half turn) showed significant decrease of penicillin amidase (PA) activity, suggesting the CRP as a major activator. In this study, the evidence fur the importance of spacing between tandem binding sites for CRP as well as for their location related to the promoter core sequence has been provided. Involvement of integration host factor (IHF) asan additional regulatory protein in the pac gene transcription regulation was also analyzed. It is shown that activation of the pac gene transcription is elevated by IHF. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 00:58:19