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Titolo:
Inhibition of aminopeptidase N (AP-N) and urokinase-type plasminogen activator (uPA) by zinc suppresses the invasion activity in human urological cancer cells
Autore:
Ishii, K; Usui, S; Sugimura, Y; Yamamoto, H; Yoshikawa, K; Hirano, K;
Indirizzi:
Gifu Pharmaceut Univ, Lab Pharmaceut, Gifu 5028585, Japan Gifu Pharmaceut Univ Gifu Japan 5028585 Pharmaceut, Gifu 5028585, Japan Aichi Canc Ctr, Res Inst, Dept Urol, Chikusa Ku, Nagoya, Aichi 4648681, Japan Aichi Canc Ctr Nagoya Aichi Japan 4648681 u, Nagoya, Aichi 4648681, Japan Toyama Prefectural Cent Hosp, Toyama 9308550, Japan Toyama Prefectural Cent Hosp Toyama Japan 9308550 Toyama 9308550, Japan Aichi Med Univ, Dept Pathol 2, Nagakute, Aichi 4801195, Japan Aichi Med Univ Nagakute Aichi Japan 4801195 agakute, Aichi 4801195, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 3, volume: 24, anno: 2001,
pagine: 226 - 230
SICI:
0918-6158(200103)24:3<226:IOAN(A>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY ADENOCARCINOMA; SEMINAL FLUID; MATRIX; METALLOPROTEINASES; DEGRADATION; RECEPTOR; TISSUE; SERUM;
Keywords:
aminopeptidase N; urokinase-type plasminogen activator; zinc; invasion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Hirano, K Gifu Pharmaceut Univ, Lab Pharmaceut, 5-6-1 Mitahora Higashi, Gifu 5028585, Japan Gifu Pharmaceut Univ 5-6-1 Mitahora Higashi Gifu Japan 5028585
Citazione:
K. Ishii et al., "Inhibition of aminopeptidase N (AP-N) and urokinase-type plasminogen activator (uPA) by zinc suppresses the invasion activity in human urological cancer cells", BIOL PHAR B, 24(3), 2001, pp. 226-230

Abstract

Zinc is an essential heavy metal and is more abundant in human prostate and kidney than in other tissues. The effects of zinc on the invasion activity. of human prostate and renal cancer cell lines, PC-3, LNCaP and SKRC-1, were investigated in vitro, using a Transwell cell-culture chamber and Mere compared with specific protease inhibitors for MMPs, uPA and AP-N, respectively, The invasion activity of PC-3 tells was effectively suppressed by zinc and by all protease inhibitors in a dose-dependent manner. The invasion activity of LNCaP cells was almost unaffected by these inhibitors. In SKRC-1cells, the invasion activity. was strongly suppressed by MP03, although a moderate inhibition by zinc and bestatin was observed. The purified AP-N activity was strongly inhibited by zinc at a concentration similar to that suppressing the invasion activity of PC-3 tells and this inhibition by zinc was apparently competitive. Although the purified uPA activity uPA was also inhibited by zinc, this inhibition was uncompetitive. AP-N was expressed abundantly on the membrane fraction of PC-3 cells among these cells tested, while its expression on the membrane fraction of SKRC-1 cells was weaker than that of PC-3 cells. The expression of uPA was also highest on the membrane fraction of PC-3 cells, These results suggest that AP-N and uPA may he involved in the invasion of human prostate cancer cells and that zinc probably participates in the invasion and metastasis of cancer cells through the regulation of the enzymatic activity of AP-N and uPA in human cancerous prostate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:05:02