Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells
Autore:
Igarashi, M; Miyazawa, T;
Indirizzi:
Tohoku Univ, Grad Sch Life Sci & Agr, Chem Biodynam Lab, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 ab, Sendai, Miyagi 9818555, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 2-3, volume: 1530, anno: 2001,
pagine: 162 - 171
SICI:
1388-1981(20010226)1530:2-3<162:TGIEOC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN TUMOR-CELLS; DIENOIC DERIVATIVES; MURINE KERATINOCYTES; CANCER-CELLS; RAT-LIVER; PROLIFERATION; APOPTOSIS; MODULATION; EXPRESSION; CULTURE;
Keywords:
conjugated linoleic acid; linoleic acid; lipid peroxidation; phospholipid hydroperoxide; cell growth inhibition; hepatoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Miyazawa, T Tohoku Univ, Grad Sch Life Sci & Agr, Chem Biodynam Lab, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 Miyagi 9818555, Japan
Citazione:
M. Igarashi e T. Miyazawa, "The growth inhibitory effect of conjugated linoleic acid on a human hepatoma cell line, HepG2, is induced by a change in fatty acid metabolism, but not the facilitation of lipid peroxidation in the cells", BBA-MOL C B, 1530(2-3), 2001, pp. 162-171

Abstract

We investigated the growth inhibitory effect of conjugated linoleic acid (CLA) on HepG2 (human hepatoma cell line), exploring whether the inhibitory action occurs via lipid peroxidation in the cells. When the cells were incubated up to 72 h with 5-40 muM of CLA (a mixture of 9c,11t-18:2 and 10t,12c-18:2), cell proliferation was clearly inhibited in a dose and time dependent manner but such an inhibition was not confirmed with linoleic acid (LA). In order to evaluate the possible contribution of lipid peroxidation exerted by CLA to cell growth inhibition, cl-tocopherol (5-20 muM) and BHT (1-10muM) as potent antioxidants were added to the medium with CLA (20 muM), which did not restore cell growth at all. Furthermore, after 72 h incubation,the membranous phospholipid hydroperoxide formation in the CLA-supplemented cells was suppressed respectively to 25% and 50% of that in LA-supplemented cells and control cells. No difference was observed by a conventional lipid peroxide assay, the TEA test, between CLA-supplemented cells and LA-supplemented cells. Although the cellular lipid peroxidation was not stimulated, lipid contents (triacylglycerol, total cholesterol and free cholesterol)and fatty acid contents (palmitic acid, palmitoleic acid and stearic acid)markedly increased in CLA-supplemented cells compared with LA-supplementedand control cells. Moreover, supplementation with 20 muM LA and 20 muM arachidonic acid profoundly interfered with the inhibitory effect of CLA in HepG2. These results suggest that the growth inhibitory effect of CLA on HepG2 is due to changes in fatty acid metabolism but not to lipid peroxidation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:03:31