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Titolo:
Identification and tissue distribution of the novel human cytochrome P4502S1 (CYP2S1)
Autore:
Rylander, T; Neve, EPA; Ingelman-Sundberg, M; Oscarson, M;
Indirizzi:
Karolinska Inst, Inst Environm Med, Div Mol Toxicol, SE-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17177 ol, SE-17177 Stockholm, Sweden
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 281, anno: 2001,
pagine: 529 - 535
SICI:
0006-291X(20010223)281:2<529:IATDOT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTENTIAL FUNCTIONAL-SIGNIFICANCE; MAMMARY EPITHELIAL-CELLS; METABOLIC-ACTIVATION; MOLECULAR-CLONING; EXPRESSION; SUBFAMILY; LOCALIZATION; DATABASE; PROTEIN; ENZYMES;
Keywords:
extrahepatic P450; human lung; EST-cloning; drug metabolism; phylogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Rylander, T Karolinska Inst, Inst Environm Med, Div Mol Toxicol, Box 210, SE-17177 Stockholm, Sweden Karolinska Inst Box 210 Stockholm Sweden SE-17177 olm, Sweden
Citazione:
T. Rylander et al., "Identification and tissue distribution of the novel human cytochrome P4502S1 (CYP2S1)", BIOC BIOP R, 281(2), 2001, pp. 529-535

Abstract

With the aid of the htgs and dbEST databases, a novel cytochrome P450 cDNAwas found by homology searches, and the corresponding gene was identified on chromosome 19. Nested PCR was used to amplify a full-length sequence of 1515 bp. The predicted 504 amino acid sequence displays 38-49% identity with CYP2 family members and the protein was designated CYP2S1. mRNA dot blot analysis demonstrated high expression levels in trachea, lung, stomach, small intestine, and spleen. The expression pattern was confirmed by Northern blot, which also revealed a single transcript of approximately 2.4 kb. Western blot analysis, using an antiserum directed against the C-terminus of the enzyme, detected a protein in human lung with the same mobility as recombinant CYP2S1. Subcellular fractionation and immunostaining revealed that CYP2S1 was localized in the endoplasmic reticulum. We conclude that CYP2S1 represents a novel abundantly expressed human P450 with potential importance for extrahepatic xenobiotic metabolism. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 08:59:41