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Titolo:
Sensitivity of heliothine larvae to wild-type and recombinant strains of asingle nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus
Autore:
Ignoffo, CM; Wong, JFH; McIntosh, AH; McCutchen, WF; Grasela, JJ; Goodman, CL; Saathoff, SG;
Indirizzi:
USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA USDA ARS Columbia MO USA 65203 ol Insects Res Lab, Columbia, MO 65203 USA Dupont Co, Stine Haskell Res Ctr, Agr Prod, Newark, DE 19714 USA Dupont Co Newark DE USA 19714 ell Res Ctr, Agr Prod, Newark, DE 19714 USA
Titolo Testata:
APPLIED ENTOMOLOGY AND ZOOLOGY
fascicolo: 1, volume: 36, anno: 2001,
pagine: 127 - 136
SICI:
0003-6862(200102)36:1<127:SOHLTW>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-POLYHEDROSIS-VIRUS; AUTOGRAPHA-CALIFORNICA LEPIDOPTERA; LEIURUS-QUINQUESTRIATUS-HEBRAEUS; INSECT-SPECIFIC TOXIN; HELICOVERPA-ZEA; NOCTUIDAE; MORTALITY; GENE; EXPRESSION; NEUROTOXIN;
Keywords:
Heliothis/Helicoverpa-SNPV; Autographa-MNPV; baculoviruses; recombinants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ignoffo, CM USDA ARS, Biol Control Insects Res Lab, 1503 S Providence, Columbia, MO 65203 USA USDA ARS 1503 S Providence Columbia MO USA 65203 MO 65203 USA
Citazione:
C.M. Ignoffo et al., "Sensitivity of heliothine larvae to wild-type and recombinant strains of asingle nucleocapsid (HzSNPV) and a multiple nucleocapsid (AcMNPV) baculovirus", APPL ENT ZO, 36(1), 2001, pp. 127-136

Abstract

Mid-stadium larvae of Helicoverpa tea were equally susceptible to either the wild-type (Wt) or recombinant (RcHz-Lqh) strain of HzSNPV. There was, however, a significant difference (1.8-fold) in the rate of mortality betweenthe two strains. The LT50 for the Wt and Re strain was 3.4+/-0.1 days and 1.9+/-0.2 days, respectively. In contrast, there was a significant difference in both the rate and extent of mortality of mid-stadium larvae of H. virescens fed either the Wt or Re strain of HzSNPV (LC50: Wt. 11.4 +/- 0.6 OB/cm(2); Re, 42.2 +/- 8.2 OB/cm(2). LT50: Wt, 4.1+/-0.1 days; Re, 2.8+/-0.2 days). An 8-fold increase in the viral rate of the WtHzSNPV was needed (800 OB/mm(2)) to match the effectiveness of the RcHzLqh strain at 100 OB/cm(2). Increasing the RcHzLqh rate above 100 OB/cm(2) did not significantly reduce the LT50 or larval feeding as measured by frass weight. All viral rates of the Re strains significantly reduced (ca. 6- to 12-fold) mid-stadium larval feeding over that of the control. Differences between the Re and Wt strains of HzSNPV, using late-stadium H. zea larvae, showed the same tendency as that using mid-stadium larvae, but the differences were not as great (< 2-fold). Mid-stadium larvae of H. virescens were ca. 3-fold more susceptibleto RcAcLqh than to either the WtAc or RcAcAa. Both RcAcL.qh and RcAcAa strains killed larvae quicker(1.5- to 1.7-fold) and reduced feeding more (1.6-to 1.9-fold) than the WtAc strain. A combination of RcAcLqh and RcAcAa at 5 OB/cm(2) had no additive effect on mortality of H. virescens larvae and the combination was less effective than either Re strain at 10 OB/cm(2). Unexpectedly, the known level of susceptibility of semi-permissive larvae of H. zea to WtAcMNPV, as measured by percent mortality, LT50, and feeding, wassignificantly enhanced (ca. 2-fold) when H. tea larvae were fed the RcHzLqh strain. In contrast, expression of the Lqh-toxin by RcHzLqh did not significantly increase the susceptibility of H. subflexa, larvae previously reported as being highly resistant to WtHzSNPV.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:32:09