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Titolo:
Solid-phase capture of proteins, spores, and bacteria
Autore:
Weimer, BC; Walsh, MK; Beer, C; Koka, R; Wang, X;
Indirizzi:
Utah State Univ, Ctr Microbe Detect & Physiol, Dept Nutr & Food Sci, Logan, UT 84322 USA Utah State Univ Logan UT USA 84322 t Nutr & Food Sci, Logan, UT 84322 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 3, volume: 67, anno: 2001,
pagine: 1300 - 1307
SICI:
0099-2240(200103)67:3<1300:SCOPSA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI O157-H7; LINKED-IMMUNOSORBENT-ASSAY; IN-GROUND BEEF; IMMUNOMAGNETIC SEPARATION; RAPID DETECTION; APPLE JUICE; ENZYME IMMUNOASSAYS; SORBITOL-MACCONKEY; FILTER TECHNIQUE; FLOW-CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Weimer, BC Utah State Univ, Ctr Microbe Detect & Physiol, Dept Nutr & FoodSci, 8700 Old Main Hill, Logan, UT 84322 USA Utah State Univ 8700 Old MainHill Logan UT USA 84322 84322 USA
Citazione:
B.C. Weimer et al., "Solid-phase capture of proteins, spores, and bacteria", APPL ENVIR, 67(3), 2001, pp. 1300-1307

Abstract

Current methods for the detection of pathogens in food and water samples generally require a preenrichment step that allows selective enrichment of the test organism. The objective of this research was to eliminate an enrichment step to allow detection of bacteria directly in food and water samplesin 30 min. A high-flow-rate, fluidized bed to capture and concentrate large (bacteria and spores) and small (protein) molecules was developed. This format, ImmunoFlow, is volume independent and uses large beads (greater than3 mm in diameter) when capturing bacteria to prevent sample clogging when testing food samples. Detection of bound targets was done using existing enzyme-linked immunosorbent assay (ELISA) protocols. Four antibodies (anti- Escherichia coli O157:H7, -Bacillus globigii, -bovine serum albumin [BSA], and -ovalbumin [OVA]) were covalently coupled to various glass and ceramic beads. Very small amounts of BSA (<1 ng) and OVA (0.2 to 4.0 <mu>g) were detected. Various industrial and environmental samples were used to observe the effect of the sample composition on the capture of anti-B. globigii and anti-E. coli O157:H7 modified beads. The lower limit of detection for both E. coli O157:H7 and B. globigii was 1 spore/cell independent of the sample size. The activity of anti-B. globigii modified beads declined after 3 days. Anti-E. coli O157:H7 modified beads declined in the capture ability after 2 days in various storage buffers. Storage temperature (4 and 25 degreesC) did not influence the stability. The ImmunoFlow technology is capable of capturing bacteria and spores directly from samples, with subsequent detection in an ELISA format in 30 min.

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Documento generato il 25/11/20 alle ore 16:57:51