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Titolo:
Chromate reduction by a pseudomonad isolated from a site contaminated withchromated copper arsenate
Autore:
McLean, J; Beveridge, TJ;
Indirizzi:
Univ Guelph, Coll Biol Sci, Dept Microbiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 icrobiol, Guelph, ON N1G 2W1, Canada
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 3, volume: 67, anno: 2001,
pagine: 1076 - 1084
SICI:
0099-2240(200103)67:3<1076:CRBAPI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEXAVALENT CHROMIUM; BACILLUS-SUBTILIS; ENTEROBACTER-CLOACAE; ENZYMATIC REDUCTION; CELL-WALL; RESISTANCE; PLASMID; FLUORESCENS; KINETICS; STRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Beveridge, TJ Univ Guelph, Coll Biol Sci, Dept Microbiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 ph, ON N1G 2W1, Canada
Citazione:
J. McLean e T.J. Beveridge, "Chromate reduction by a pseudomonad isolated from a site contaminated withchromated copper arsenate", APPL ENVIR, 67(3), 2001, pp. 1076-1084

Abstract

A.pseudomonad (CRB5) isolated from a decommissioned wood preservation sitereduced toxic chromate [Cr(VI)] to an insoluble Cr(III) precipitate under aerobic and anaerobic conditions. CRB5 tolerated up to 520 mg of Cr(VI) liter(-1) and reduced chromate in the presence of copper and arsenate, Under anaerobic conditions it also reduced Co(III) and U(VI), partially internalizing each metal, Metal precipitates were also found on the surface of the outer membrane and (sometimes) on a capsule. The results shelved that chromate reduction by CRB5 was mediated by a soluble enzyme that was largely contained in the cytoplasm but also found outside of the cells. The crude reductase activity in the soluble fraction showed a K-m of 23 mg liter(-1)(437 muM) and a V-max of 0.98 mg of Cr h(-1) mg of protein(-1) (317 nmol min(-1) mg of protein(-1)). Minor membrane-associated Cr(VI) reduction under anaerobiosis may account for anaerobic reduction of chromate under nongrowth conditions fifth an organic electron donor present. Chromate reduction under both aerobic and anaerobic conditions may be a detoxification strategy far thebacterium which could be exploited to bioremediate chromate-contaminated or other toxic heavy metal-contaminated environments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:54:05