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Titolo:
Molecular cloning and identification of bottle-nosed dolphin p40(phox), p47(phox) and p67(phox)
Autore:
Inoue, Y; Itou, T; Jimbo, T; Sakai, T; Ueda, K; Imajoh-Ohmi, S;
Indirizzi:
Nihon Univ, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, Fujisawa, Kanagawa 2528510, Japan Nihon Univ Fujisawa Kanagawa Japan 2528510 isawa, Kanagawa 2528510, Japan Nihon Univ, Coll Bioresource Sci, Dept Appl Biol Sci, Fujisawa, Kanagawa 2528510, Japan Nihon Univ Fujisawa Kanagawa Japan 2528510 isawa, Kanagawa 2528510, Japan Univ Tokyo, Inst Med Sci, Dept Bacterial Infect, Minato Ku, Tokyo 108, Japan Univ Tokyo Tokyo Japan 108 Bacterial Infect, Minato Ku, Tokyo 108, Japan
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 1, volume: 78, anno: 2001,
pagine: 21 - 33
SICI:
0165-2427(20010110)78:1<21:MCAIOB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHAGOCYTE NADPH OXIDASE; TURSIOPS-TRUNCATUS; FUNCTIONAL EXPRESSION; CYTOSOLIC COMPONENT; MOUSE HOMOLOG; KILLER WHALE; ORCINUS-ORCA; ACTIVATION; PROTEIN; SUBUNIT;
Keywords:
bottle-nosed dolphin; p40(phox); p47(phox); p67(phox); Superoxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sakai, T Nihon Univ, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, 1866 Kameino, Fujisawa, Kanagawa 2528510, Japan Nihon Univ 1866 Kameino Fujisawa Kanagawa Japan 2528510 10, Japan
Citazione:
Y. Inoue et al., "Molecular cloning and identification of bottle-nosed dolphin p40(phox), p47(phox) and p67(phox)", VET IMMUNOL, 78(1), 2001, pp. 21-33

Abstract

The bottle-nosed dolphin NADPH oxidase cytosolic components, p40(phox), p47(phox) and p67(phox) cDNA's were cloned from mitogen stimulated peripheralwhite blood cell mRNA utilizing the reverse transcription-polymerase chainreaction. The sequences of these cDNAs showed that dolphin p40(phox), p47(phox) and p67(phox): clones contained open reading frames encoding predicted polypeptides of 339, 391 and 526 amino acids, respectively. Analysis of the p47(phox) and p67(phox) amino acid sequences showed two potential Src homology three domains and p40(phox) one. Comparison of the deduced amino acids showed that dolphin p40(phox) sequence shared 88.8% similarity with the human p40(phox), that dolphin p47(phox) sequence shared 87.7% similarity with the bovine p47(phox), and that dolphin-p67(phox) shared 88.1% similaritywith the bovine p67(phox). Western blot analysis using anti-human p40(phox), p47(phox) and p67(phox) antibodies demonstrated that dolphin neutrophil possesses p40(phox), p47(phox) and p67(phox) with similar molecular masses and structures, to each counterpart in human neutrophils, except for the p67(phox) COOH-terminus. These results suggest that dolphin NADPH oxidase cytosolic components have functional activities equivalent to those of human. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:33:16