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Titolo:
Fibrin clue as matrix for cultured autologous urothelial cells in urethralreconstruction
Autore:
Bach, AD; Bannasch, H; Galla, TJ; Bittner, KM; Stark, GB;
Indirizzi:
Univ Freiburg, Med Ctr, Dept Plast & Hand Surg, D-79106 Freiburg, Germany Univ Freiburg Freiburg Germany D-79106 d Surg, D-79106 Freiburg, Germany Univ Freiburg, Med Ctr, Valley Tissue Engn Ctr, D-79106 Freiburg, Germany Univ Freiburg Freiburg Germany D-79106 gn Ctr, D-79106 Freiburg, Germany
Titolo Testata:
TISSUE ENGINEERING
fascicolo: 1, volume: 7, anno: 2001,
pagine: 45 - 53
SICI:
1076-3279(200102)7:1<45:FCAMFC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; HUMAN KERATINOCYTES; BLADDER MUCOSA; FIBRONECTIN; COLLAGEN; VIVO; GLUE; MIGRATION; COMPOSITE; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Bach, AD Univ Freiburg, Med Ctr, Dept Plast & Hand Surg, Hugstetter Str 55, D-79106Freiburg, Germany Univ Freiburg Hugstetter Str 55 Freiburg Germany D-79106 Germany
Citazione:
A.D. Bach et al., "Fibrin clue as matrix for cultured autologous urothelial cells in urethralreconstruction", TISSUE ENG, 7(1), 2001, pp. 45-53

Abstract

In the present study, we have established a technique to create an artificial urethra in a rat animal model by transplantation of in vitro-expanded urothelial cells onto an in vivo-prefabricated tube formation using tissue engineering methods. Urothelial cells from isogenic rats were harvested for culture. A silicon catheter was used to induce a connective tissue capsule-tube formation underneath the abdominal skin. Two weeks later, the cultivated urothelial cells were seeded onto the lumen of this tube using fibrin glue as delivery matrix. The histomorphological and immunohistochemical studies revealed a viable multilayered urothelium, lining the inner surface of the prior formed connective tissue tube-formation 4 weeks after grafting thecells, We have shown that cultured and in vitro-expanded urothelial cells can be successfully reimplanted onto a prefabricated tube-like structure using fibrin glue as a delivery matrix and native cell expansion vehicle. Theresults suggest that the creation of an artificial urethra may be achievedin vivo using tissue engineering methods, showing potential for urethral reconstruction and providing autologous urothelium for reconstructive surgery in the genitourinary tract.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:56:09