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Titolo:
Reconstruction of the heteroparental diploid condition in rabbit zygotes by nuclear transfer
Autore:
Escriba, MJ; Garcia-Ximenez, F;
Indirizzi:
Univ Politecn Valencia, Dept Ciencia Anim, Anim Reprod & Biotechnol Lab, E-46071 Valencia, Spain Univ Politecn Valencia Valencia Spain E-46071 b, E-46071 Valencia, Spain
Titolo Testata:
THERIOGENOLOGY
fascicolo: 3, volume: 55, anno: 2001,
pagine: 771 - 784
SICI:
0093-691X(20010201)55:3<771:ROTHDC>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
FULL-TERM DEVELOPMENT; IN-VITRO DEVELOPMENT; PARTHENOGENETIC DEVELOPMENT; ENUCLEATED OOCYTES; MOUSE EMBRYOS; TRANSPLANTATION; ACTIVATION; GENOME; BEHAVIOR; SEQUENCE;
Keywords:
haploid embryo; hemizygous; nuclear transfer; rabbits;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Escriba, MJ Univ Politecn Valencia, Dept Ciencia Anim, Anim Reprod & Biotechnol Lab, E-46071 Valencia, Spain Univ Politecn Valencia Valencia Spain E-46071 alencia, Spain
Citazione:
M.J. Escriba e F. Garcia-Ximenez, "Reconstruction of the heteroparental diploid condition in rabbit zygotes by nuclear transfer", THERIOGENOL, 55(3), 2001, pp. 771-784

Abstract

Studies on genomic imprinting showed that parental genomes have complementary roles during embryogenesis, are both essential and need to be synchronized in their embryonic stage for successful development to term. To our knowledge, these studies have not been performed in species other than mice Westudied the in vitro and in vivo development of reconstructed zygotes by combining female haploid nuclear donors and androgenetic hemizygous recipients Haploid donor embryos at the 8- or 32-cell stage were obtained from electroactivated young rabbit ova (eight pulses maximum, consisting of 0 6 kVcm(-1) for 60 mu sec each, 38 min apart) which were further cultured for 24 hor 32 h Couplets formed by both the haploid male hemizygous recipients andhaploid female donor cells were electrofused (2.2 kVcm(-1) for 60 mu sec duration each, 30 min apart) and their. nuclear configuration determined. 122 Of those fused (43%: 122/286) were diploid Reconstructed diploid zygotes developed in vitro up to the compacted morula, blastocyst and hatched stages (1/8-nucler 50%, 18% and 9% vs. 1/32-nuclei: 47%, 25% and 19%; P > 0.05),respectively. In embryo transfer assays, both 1/32-reconstructed zygotes and control, non-manipulated zygotes were transferred to synchronized does Four live reconstructed fetuses (4/49: 81% survival rate) and five in regression stage (9/49: 18% implantation rate) were observed on Day 21 post-ovulation, whereas from control zygotes, ii fetuses were alive(11/53 21% fetal survival rate) and 2 degenerated (13/53 24 5% implantation rate). Similar results were obtained from a final experiment, in which development was allowed to progress lo term Six live rabbit pups derived from experimentally reconstructed zygotes (11%: 6/54) and three fetuses In regression stage were obtained; values slightly lower than those derived from non-manipulated and transferred control zygotes (18% 9/50, live born rate). (C) 2001 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:18:53