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Titolo:
Development and characterisation of simple sequence repeat (SSR) markers for white clover (Trifolium repens L.)
Autore:
Kolliker, R; Jones, ES; Drayton, MC; Dupal, MP; Forster, JW;
Indirizzi:
La Trobe Univ, Agr Victoria, Ctr Plant Biotechnol, Bundoora, Vic 3083, Australia La Trobe Univ Bundoora Vic Australia 3083 , Bundoora, Vic 3083, Australia
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 2-3, volume: 102, anno: 2001,
pagine: 416 - 424
SICI:
0040-5752(200102)102:2-3<416:DACOSS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSATELLITE MARKERS; GENETIC-DISTANCE; DNA MARKERS; PLANTS; MAP; CONSTRUCTION; MEDICAGO; AFLP; RAPD; IDENTIFICATION;
Keywords:
simple sequence repeats; SSR-enriched libraries; molecular marker; Trifolium repens; cross-species amplification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Forster, JW La Trobe Univ, Agr Victoria, Ctr Plant Biotechnol, Bundoora, Vic 3083, Australia La Trobe Univ Bundoora Vic Australia 3083 Vic 3083, Australia
Citazione:
R. Kolliker et al., "Development and characterisation of simple sequence repeat (SSR) markers for white clover (Trifolium repens L.)", THEOR A GEN, 102(2-3), 2001, pp. 416-424

Abstract

Highly informative molecular markers, such as simple sequence repeats (SSRs), can greatly accelerate breeding programs. The aim of this study was to develop and characterise a comprehensive set of SSR markers for white clover (Trifolium repens L.), which can be used to tag genes and quantitative trait loci controlling traits of agronomic interest. Sequence analysis of 1123 clones from genomic libraries enriched for (CA)(n) repeats yielded 793 clones containing SSR loci. The majority of SSRs consisted of perfect dinucleotide repeats, only 7% being trinucleotide repeats. After exclusion of redundant sequences and SSR loci with less than 25 bp of flanking sequence, 397potentially useful SSRs remained. Primer pairs were designed for 117 SSR loci and PCR products in the expected size range were amplified from 101 loci. These markers were highly polymorphic, 88% detecting polymorphism acrossseven white clover genotypes with an average allele number of 4.8. Four primer pairs were tested in an F-2 population revealing Mendelian segregation. Successful cross-species amplification was achieved in at least one out of eight legume species for 46 of 54 primer pairs. The rate of successful amplification was significantly higher for Trifolium species when compared tospecies of other genera. The markers developed in this study not only provide valuable tools for molecular breeding of white clover but may also haveapplications in related taxa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 00:00:14