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Titolo:
Quantitative analysis of gene expressions related to inflammation in canine spastic artery after subarachnoid hemorrhage
Autore:
Aihara, Y; Kasuya, H; Onda, H; Hori, T; Takeda, J;
Indirizzi:
Tokyo Womens Med Univ, Dept Neurosurg, Shinjuku Ku, Tokyo 1628666, Japan Tokyo Womens Med Univ Tokyo Japan 1628666 njuku Ku, Tokyo 1628666, Japan Gunma Univ, Inst Mol & Cellular Regulat, Dept Cell Biol, Mol Genet Lab, Maebashi, Gumma 371, Japan Gunma Univ Maebashi Gumma Japan 371 Genet Lab, Maebashi, Gumma 371, Japan
Titolo Testata:
STROKE
fascicolo: 1, volume: 32, anno: 2001,
pagine: 212 - 217
SICI:
0039-2499(200101)32:1<212:QAOGER>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; CHRONIC CEREBROVASOSPASM; BASILAR ARTERY; EXPERIMENTAL VASOSPASM; CEREBRAL-ARTERIES; ACTIVATION; RABBITS; ICAM-1; PIGS; RATS;
Keywords:
cerebral vasospasm; cytokines; inflammation; subarachnoid hemorrhage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kasuya, H Tokyo Womens Med Univ, Dept Neurosurg, Shinjuku Ku, Kawada Cho 8-1, Tokyo 1628666, Japan Tokyo Womens Med Univ Kawada Cho 8-1 Tokyo Japan 1628666 Japan
Citazione:
Y. Aihara et al., "Quantitative analysis of gene expressions related to inflammation in canine spastic artery after subarachnoid hemorrhage", STROKE, 32(1), 2001, pp. 212-217

Abstract

Background and Purpose-The possible role of inflammatory reaction of the cerebral artery in the pathogenesis of cerebral vasospasm has been noted in recent studies. We quantitatively measured the levels of expression of genes related to inflammation in the spastic artery in a canine double-hemorrhage model. Methods-Twenty dogs were assigned to 4 groups: group D0, control; group D2, dogs killed 2 days after cisternal injection of blood; group D7, dogs given double cisternal injections of blood and killed 7 days after the first injection; and group D14. Angiography was performed twice: on the first day and before the animals were killed. Total RNA was extracted from the basilar artery. The expressions of interleukin (IL)-1 alpha, IL-6, IL-8, IL-10, tumor necrosis factor-alpha, E-secretin, fibronectin, intercellular adhesionmolecule (ICAM)-1, vascular cell adhesion molecule-1, transforming growth factor-beta, basic fibroblast growth factor, and collagen types I, III, andIV were examined with TaqMan real-time quantitative reverse transcription-polymerase chain reaction. Results-Prolonged arterial narrowing peaking on 7 day was observed. There was a significant difference in vessel caliber between D0, D2, D7, and D14 groups (P<0.0001). There were significant differences in mRNA expression inthe basilar artery for IL-1<alpha>, IL-6, IL-6, ICAM-1, and collagen type I between D0, D2, D7, and D14 groups (P=0.0079, 0.0196, 0.0040, 0.0017, and<0.0001, respectively). The average level of mRNA was highest in D7 for IL-1<alpha>, IL-6, IL-8, and ICAM-1 (17-, 16-, 131-, and 1.7-fold compared with those of D0, respectively) and in D14 for collagen type I (10.9-fold). Conclusions-Increased expression of genes related to inflammation in the spastic artery suggests that inflammatory reaction of the cerebral artery isassociated with sustained contraction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 21:12:18