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Titolo:
Gene transfer of extracellular superoxide dismutase increases superoxide dismutase activity in cerebrospinal fluid
Autore:
Nakane, H; Chu, Y; Faraci, FM; Oberley, LW; Heistad, DD;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ IowaIowa City IA USA 52242 pt Internal Med, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Pharmacol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Dept Pharmacol, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Ctr Cardiovasc, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Ctr Cardiovasc, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Radiat Res Lab, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Radiat Res Lab, Iowa City, IA 52242 USA
Titolo Testata:
STROKE
fascicolo: 1, volume: 32, anno: 2001,
pagine: 184 - 189
SICI:
0039-2499(200101)32:1<184:GTOESD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL BLOOD-VESSELS; TRANSFER IN-VIVO; SUBARACHNOID HEMORRHAGE; PERIVASCULAR TISSUE; ENDOTHELIAL-CELLS; ISCHEMIC-INJURY; MICE; HYPERTENSION; ANTIOXIDANTS; TOXICITY;
Keywords:
adenovirus; basilar artery; gene transfer; heparin; superoxide dismutase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Heistad, DD Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Med, Iowa City, IA 52242 USA
Citazione:
H. Nakane et al., "Gene transfer of extracellular superoxide dismutase increases superoxide dismutase activity in cerebrospinal fluid", STROKE, 32(1), 2001, pp. 184-189

Abstract

Background and Purpose-Copper-zinc superoxide dismutase (CuZnSOD) is expressed intracellularly, while extracellular SOD (EC-SOD) is released from cells. The purpose of this study was to determine whether gene transfer of CuZnSOD increases SOD activity predominantly in tissues, and gene transfer of EC-SOD increases SOD activity in cerebrospinal fluid (CSF). We also determined whether heparin or dextran sulfate releases EC-SOD into CSF. Methods-We injected recombinant adenoviruses expressing EC-SOD (AdEC-SOD),CuZnSOD (AdCuZnSOD), or beta -galactosidase (Ad beta -gal) into the cisterna magna of rabbits. Results-Total SOD activity in CSF was 39+/-11 U/mL (mean+/-SE) before virus injection. Three days later, total SOD activity in CSF increased to 148+/-22 U/mL after AdEC-SOD and 92+/-10 U/mL after AdCuZnSOD (P<0.05 versus AdEC-SOD), with no change after Ad<beta>-gal (49+/-5 U/mL). EC-SOD protein wasdetected in CSF after AdEC-SOD but not AdCuZnSOD or Ad beta -gal. Injection of heparin or dextran sulfate into the cisterna magna increased total SODactivity 27-fold and 32-fold over basal values, respectively, in CSF of rabbits that received AdEC-SOD. In contrast to effects in CSF, total SOD activity in basilar artery and meninges was significantly higher after AdCuZnSOD and tended to be higher after AdEC-SOD than after Ad beta -gal. Conclusions-We have developed a method for intracranial gene transfer of CuZnSOD and EC-SOD. After gene transfer, CuZnSOD was expressed mainly in tissues, and EC-SOD was released into the CSF, especially after injection of heparin or dextran sulfate. Gene transfer of different isoforms of SOD may be useful in studies of cerebral vascular physiology and pathophysiology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:17:55