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Titolo:
Stimulatory effects of compounds present in the rhizosphere on natural transformation of Acinetobacter sp BD413 in soil
Autore:
Nielsen, KM; van Elsas, JD;
Indirizzi:
Norwegian Univ Sci & Technol, Fac Chem & Biol, Dept Bot, N-7491 Trondheim,Norway Norwegian Univ Sci & Technol Trondheim Norway N-7491 91 Trondheim,Norway Plant Res Int, NL-6700 AA Wageningen, Netherlands Plant Res Int Wageningen Netherlands NL-6700 AA Wageningen, Netherlands
Titolo Testata:
SOIL BIOLOGY & BIOCHEMISTRY
fascicolo: 3, volume: 33, anno: 2001,
pagine: 345 - 357
SICI:
0038-0717(200103)33:3<345:SEOCPI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORIZONTAL GENE-TRANSFER; TRANSGENIC PLANT DNA; BACILLUS-SUBTILIS; FLUORESCENT PSEUDOMONADS; STRAIN BD413; BACTERIA; CALCOACETICUS; RESISTANCE; MICROCOSMS; ACIDS;
Keywords:
natural transformation; soil; competence; gene transfer; Acinetobacter sp; rhizosphere; root exudates;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Nielsen, KM Norwegian Univ Sci & Technol, Fac Chem & Biol, Dept Bot, N-7491 Trondheim,Norway Norwegian Univ Sci & Technol Trondheim Norway N-7491 ,Norway
Citazione:
K.M. Nielsen e J.D. van Elsas, "Stimulatory effects of compounds present in the rhizosphere on natural transformation of Acinetobacter sp BD413 in soil", SOIL BIOL B, 33(3), 2001, pp. 345-357

Abstract

The stimulation of natural transformation of Acinetobacter sp. BD413(pFG4)cells by various organic compounds naturally found in the rhizosphere of crop plants was determined in sterile and non-sterile soil. Stimulation of bacterial competence by the organic acids, amino acids, sugars and inorganicfertilizers, was monitored by the transformation frequencies obtained withadded cell lysates of Acinetobacter sp. carrying a kanamycin resistance gene (nptII). Clear differences in the capability of the organic compounds toinduce gene capture were detected. In sterile soil, most of the organic compounds used stimulated the appearance of transformants. Fewer compounds were able to induce transformation in non-sterile soil, and a high-P salt supplementation was found to be required for detectable gene transfer. The transformation frequencies in non-sterile soil were, however, if found, on average less than 10-fold reduced compared to those obtained under sterile soil conditions. Of the organic acids and amino acids applied in non-sterile soil, acetate, lactate and alanine gave the highest transformation frequencies, i.e. 1 to 2 x 10(-6). Of the sugars used, only glucose produced a few transformants in non-sterile soil (2.5 x 10(-7)). Mixtures of the organic and amino acids, or the sugars, and the high-P salts produced the highest transformation frequencies in nonsterile soil, of 4.4 to 6.6 x 10(-6). The addition of fertilizers had a less pronounced effect on the transformation frequencies. We conclude that many of the compounds exuded into the rhizosphere of agriculturally grown plants are able to stimulate natural transformation of Acinetobacter sp. BD413(pFG4) cells in sterile silt loam soil, and that the presence of both carbon sources and inorganic salts is required for detectable gene transfer in non-sterile silt loam. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:54:49