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Titolo:
Evaluation of mass discrimination effects in the quantitative analysis of polydisperse polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using uniform oligostyrenes
Autore:
Shimada, K; Lusenkova, MA; Sato, K; Saito, T; Matsuyama, S; Nakahara, H; Kinugasa, S;
Indirizzi:
Natl Inst Mat & Chem Res, Dept Analyt Chem, Macromol Characterizat Grp, Tsukuba, Ibaraki 3058565, Japan Natl Inst Mat & Chem Res Tsukuba Ibaraki Japan 3058565 aki 3058565, Japan
Titolo Testata:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
fascicolo: 4, volume: 15, anno: 2001,
pagine: 277 - 282
SICI:
0951-4198(2001)15:4<277:EOMDEI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALDI-TOF; SYNTHETIC-POLYMERS; CHROMATOGRAPHY; DISTRIBUTIONS; ISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Kinugasa, S Natl Inst Mat & Chem Res, Dept Analyt Chem, Macromol Characterizat Grp, 1-1 Higashi, Tsukuba, Ibaraki 3058565, Japan Natl Inst Mat & ChemRes 1-1 Higashi Tsukuba Ibaraki Japan 3058565
Citazione:
K. Shimada et al., "Evaluation of mass discrimination effects in the quantitative analysis of polydisperse polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using uniform oligostyrenes", RAP C MASS, 15(4), 2001, pp. 277-282

Abstract

Mass discrimination effects in matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry (MALDI-TOFMS) were quantitatively investigated using equiweight and equimolar mixtures of uniform polystyrene (PS) oligomers. Uniform PS oligomers were separated by preparative super-criticalfluid chromatography (SFC) from commercial standard PS samples. The separated PS oligomers, with degrees of polymerization n = 2-25, have absolutely no molecular weight distributions. Equiweight and equimolar mixtures of uniform PS oligomers were accurately prepared by weighing by microbalance, andtheir spectra were recorded using a MALDI-TOF mass spectrometer. In the lower molecular weight region (less than about 10(3)) the oligomers with lower molecular weights give lower mass spectral, intensities, with no correlation with laser power. In contrast higher laser powers yield a decrease of mass spectral intensities in the higher molecular weight region. These results clearly show that mass discrimination effects occur at lower and higher molecular weights depending on the laser power, and provide quantitative information about the discrimination. Using the data on equiweight and equimolar mixtures of PS oligomers, it was possible to calibrate the MALDI-TOF mass spectral data for an analysis of molecular weight distribution of a standard monodisperse PS sample with number-averaged molecular weight of 103, and to compare it with the molecular weight distribution measured by analytical SFC. The result from the calibrated MALDI-TOF mass spectrum, however, does not agree perfectly with that from the SFC results, because undetectable peaks in MALDI-TOF mass spectra at lower and higher molecular weights could not be included in the calibration of peak intensities. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:27:35