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Titolo:
Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine-treated maize roots
Autore:
De Agazio, M; Zacchini, M;
Indirizzi:
CNR, Ist Biochim & Ecofisiol Vegetali, I-00016 Monterotondo, Italy CNR Monterotondo Italy I-00016 iol Vegetali, I-00016 Monterotondo, Italy
Titolo Testata:
PLANT CELL AND ENVIRONMENT
fascicolo: 2, volume: 24, anno: 2001,
pagine: 237 - 244
SICI:
0140-7791(200102)24:2<237:DAHPTP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; K+ UPTAKE; SPINACH-CHLOROPLASTS; OXIDATIVE STRESS; PLASMA-MEMBRANE; SALT STRESS; POLYAMINE; TOLERANCE; SEEDLINGS; SEGMENTS;
Keywords:
Zea mays L; APX activity; APX m-Rna; dimethylthiourea; root; spermidine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: De Agazio, M CNR, Ist Biochim & Ecofisiol Vegetali, Via Salaria Km 29-300,I-00016 Monterotondo, Italy CNR Via Salaria Km 29-300 Monterotondo Italy I-00016 , Italy
Citazione:
M. De Agazio e M. Zacchini, "Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine-treated maize roots", PL CELL ENV, 24(2), 2001, pp. 237-244

Abstract

Inhibition of root growth and accumulation of putrescine caused by exogenous spermidine in roots of maize seedlings (Zea mays L., cv Samodek) were partially prevented by a concomitant treatment with dimethylthiourea (DMTU), that traps H2O2 produced from spermidine by the activity of polyamine oxidase (PAO) in the apoplast. Treatment with spermidine caused a strong increase of ascorbate peroxidase (APX) gene expression, that was induced to a lesser extent by removing spermidine-generated H2O2 by DMTU. Over-expression ofAPX was associated with increased APX activity in spermidine-treated seedlings whereas the addition of DMTU to spermidine completely prevented spermidine-induced increase of APX activity. Thus, DMTU permitted the demonstration that exogenous spermidine supplied to maize seedlings causes an oxidative stress and induces APX, a key enzyme of the antioxidant defence mechanism, through H2O2, a spermidine catabolic product.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:39:27