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Titolo:
Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in arabidopsis cell culture
Autore:
Takahashi, S; Katagiri, T; Hirayama, T; Yamaguchi-Shinozaki, K; Shinozaki, K;
Indirizzi:
RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan RIKEN Tsukuba Ibaraki Japan 3050074 Lab, Tsukuba, Ibaraki 3050074, Japan Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan Univ TsukubaTsukuba Ibaraki Japan 3058572 sukuba, Ibaraki 3058572, Japan Minist Agr Forestry & Fisheries, Japan Int Res Ctr Agr Sci, Biol ResourcesDiv, Tsukuba, Ibaraki 3050851, Japan Minist Agr Forestry & Fisheries Tsukuba Ibaraki Japan 3050851 0851, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 2, volume: 42, anno: 2001,
pagine: 214 - 222
SICI:
0032-0781(200102)42:2<214:HSIARA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPASE-C; GENE-EXPRESSION; LOW-TEMPERATURE; PLANT-CELLS; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; SIGNAL-TRANSDUCTION; WATER-STRESS; SALT STRESS; THALIANA; DROUGHT;
Keywords:
Arabidopsis thaliana; hyperosmotic stress; inositol 1,4,5-trisphosphate; phosphoinositide-specific phospholipase C; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Shinozaki, K RIKEN, Tsukuba Inst, Plant Mol Biol Lab, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan RIKEN 3-1-1 Koyadai Tsukuba Ibaraki Japan 3050074 0074, Japan
Citazione:
S. Takahashi et al., "Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in arabidopsis cell culture", PLANT CEL P, 42(2), 2001, pp. 214-222

Abstract

Phospholipid metabolism is involved in hyperosmotic-stress responses in plants. To investigate the role of phosphoinositide-specific phospholipase C (PI-PLC)-a key enzyme in phosphoinositide turnover-in hyperosmotic-stress signaling, we analyzed changes in inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) content in response to hyperosmotic shock or salinity in Arabidopsis thaliana T87 cultured cells. Within a few s, a hyperosmotic shock, caused by mannitol, NaCl, or dehydration, induced a rapid and transient increase in Ins(1,4,5)P-3. However, no transient increase was detected in cells treated with ABA, Neomycin and U73122, inhibitors of PI-PLC, inhibited the increase in Ins(1,4,5)P-3 caused by the hyperosmotic shock. A rapid increase in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P-2) in response to the hyperosmotic shock also occurred, but the rate of increase was much slower than that of Ins(1,4,5)P-3. These findings indicate that the transient Ins(1,4,5)P-3 production was due to the activation of PI-PLC in response to hyperosmotic stress. PI-PLC inhibitors also inhibited hyperosmotic stress-responsive expression of some dehydration-inducible genes, such as rd29A (lti78/cor78) and rd17 (cor47), that are controlled by the DRE/CRT cis-acting element but did not inhibit hyperosmotic stress-responsive expression of ABA-inducible genes, such as rd20. Taken together, these results suggest the involvement of PI-PLC and Ins(1,4,5)P-3 in an ABA-independent hyperosmotic-stress signal transduction pathway in higher plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 05:47:38