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Titolo:
Transcriptional expression characteristics and subcellular localization ofADP-glucose pyrophosphorylase in the oil plant Perilla frutescens
Autore:
Choi, SB; Kim, KH; Kavakli, IH; Lee, SK; Okita, TW;
Indirizzi:
Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 ol Chem, Pullman, WA 99164 USA Natl Inst Agr Sci & Technol, Div Biochem, Rural Dev Adm, Suweon 441707, South Korea Natl Inst Agr Sci & Technol Suweon South Korea 441707 41707, South Korea
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 2, volume: 42, anno: 2001,
pagine: 146 - 153
SICI:
0032-0781(200102)42:2<146:TECASL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; ZEA-MAYS L; CDNA CLONES; ADPGLUCOSE PYROPHOSPHORYLASE; MOLECULAR CHARACTERIZATION; BARLEY ENDOSPERM; MAIZE ENDOSPERM; CLONING; ISOFORMS; GENE;
Keywords:
ADP-glucose pyrophosphorylase; BT2; GFP localization; Perilla frutescens; plastids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Okita, TW Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 ullman, WA 99164 USA
Citazione:
S.B. Choi et al., "Transcriptional expression characteristics and subcellular localization ofADP-glucose pyrophosphorylase in the oil plant Perilla frutescens", PLANT CEL P, 42(2), 2001, pp. 146-153

Abstract

Three ADP-glucose pyrophosphorylase clones were isolated from the cotyledon cDNA library of the oil plant, Perilla frutescens, and their intracellular localization investigated. Two of three cDNAs (PfagpS1 and PfagpS2) were homologous to the catalytic small subunit of AGPases found in other plants,while the third clone (PfagpL) was highly similar to the large subunit type. Transcripts for PfagpS1 and PfagpS2 were observed in both photosyntheticand non-photosynthetic tissue, showing the highest expression in the stem,while PfagpL transcripts were abundantly expressed in stem and cotyledon, To evaluate the subcellular localization of PfagpS2. and PfagpL as well as the maize BT2, N-terminus-GFP DNA fusion were constructed and transformed into tobacco plants, Immunoblot analysis showed that the expressed PfagpS2- and PfagpL-GFP fusions were targeted to the plastid in the heterologous tobacco system whereas the BT2-GFP remained intact, suggesting a cytoplasmic location, These intracellular assignments were confirmed by direct confocal microscopic examination. GFP signals were localized to the cytoplasm as well as in the nucleus in BT2-GFP plants, and to the plastids in PfagpS2- and PfagpL-GFP plants, Our results indicate that Perilla cotyledons contain multiple AGPase subunits, of which at least two isoforms and very likely the third, are plastidial in nature.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:23:03