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Titolo:
Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot
Autore:
Jimenez, VM; Bangerth, F;
Indirizzi:
Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, GermanyUniv Hohenheim Stuttgart Germany D-70593 370, D-70593 Stuttgart, Germany
Titolo Testata:
PHYSIOLOGIA PLANTARUM
fascicolo: 3, volume: 111, anno: 2001,
pagine: 389 - 395
SICI:
0031-9317(200103)111:3<389:EHLIEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED SOMATIC EMBRYOGENESIS; CELL-SUSPENSION CULTURE; X HORTORUM BAILEY; DAUCUS-CAROTA L; HYPOCOTYL CULTURES; ABSCISIC-ACID; PURINE METABOLISM; EXOGENOUS AUXINS; THIDIAZURON; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bangerth, F Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 Stuttgart, Germany
Citazione:
V.M. Jimenez e F. Bangerth, "Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot", PHYSL PLANT, 111(3), 2001, pp. 389-395

Abstract

Carrot (Daucus carota L. F1 hybrid Starca) excised hypocotyls were cultured on Murashige and Skoog medium with and without 2,4-dichlorophenoxy aceticacid (2,4-D) to determine the effect of this plant growth regulator on their further development and their endogenous hormone levels, Culture in the absence of 2,4-D stimulated root development at one end of the hypocotyl segments and increased the endogenous bevels of free indole-3-acetic acid (IAA), zeatin/zeatin riboside and N-6(Delta (2)-isopentenyl) adenine/N-6(Delta(2)-isopentenyl) adenosine, as determined by radioimmunoassay. On the other hand, the presence of 2,4-D in the culture medium promoted callus induction and proliferation, together with abscisic acid (ABA) accumulation, in the hypocotyl segments during the first weeks of culture. When the callus segments generated in the hypocotyl sections cultured in the presence of 2,4-Dwere cultivated further, the development of two callus types was observed,one composed of preglobular and globular embryos and the other translucent, watery and lacking any sign of organisation. The embryos of the first type germinated when callus segments were transferred to regeneration conditions, while no change was observed when the second type was induced to regenerate, Higher levels of free IAA and ABA were obtained in the embryogenic calli when compared to the non-embryogenic, while no differences were observed among callus types in the other hormones evaluated. The possible role of the different plant hormones during induction of somatic embryogenesis is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 09:52:26