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Titolo:
Phenotypic changes of T-lymphocyte subsets induced by interleukin-12 and interleukin-15 in umbilical cord vs. adult peripheral blood mononuclear cells
Autore:
Lin, SJ; Chao, HC; Yan, DC;
Indirizzi:
Chang Gung Univ, Chang Gung Childrens Hosp, Dept Pediat, Div Asthma Allergy & Rheumatol, Taoyuan, Taiwan Chang Gung Univ Taoyuan Taiwan hma Allergy & Rheumatol, Taoyuan, Taiwan
Titolo Testata:
PEDIATRIC ALLERGY AND IMMUNOLOGY
fascicolo: 1, volume: 12, anno: 2001,
pagine: 21 - 26
SICI:
0905-6157(200102)12:1<21:PCOTSI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT CELLULAR CYTOTOXICITY; HEMATOPOIETIC STEM-CELLS; NATURAL-KILLER ACTIVITY; CYTOKINE PRODUCTION; ANTITUMOR-ACTIVITY; ACTIVATED CORD; IN-VITRO; IL-12; TRANSPLANTATION; EXPRESSION;
Keywords:
cord blood; T cell; IL-12; IL-15; pediatric;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, SJ Chang Gung Univ, Chang Gung Childrens Hosp, Dept Pediat, Div Asthma Allergy & Rheumatol, 5 Fu Hsing St, Taoyuan, Taiwan Chang Gung Univ 5 Fu Hsing St Taoyuan Taiwan St, Taoyuan, Taiwan
Citazione:
S.J. Lin et al., "Phenotypic changes of T-lymphocyte subsets induced by interleukin-12 and interleukin-15 in umbilical cord vs. adult peripheral blood mononuclear cells", PEDIAT A IM, 12(1), 2001, pp. 21-26

Abstract

The decreased incidence of graft-vs.-host disease found following umbilical cord blood (CB) transplantation, and the increased susceptibility of newborns to infections, have been attributed, in part, to functional and phenotypic immaturity of neonatal T cells. We investigated the phenotypic changesof CB T cells induced by two immunoregulary cytokines, interleukin (IL)-12and IL-15, alone or in combination. Adult peripheral blood (APB) mononuclear cells (MNCs) were also tested for comparison. Prior to culture, the percentages of CD3(+) CD8(+), CD3(+) CD25(+), and CD3(+) CD56(+) cells were significantly lower in CB MNCs than in APE MNCs. IL-15, but not IL-12, significantly increased CD3(+) CD8(+) expression among the CB MNCs after 1 week ofculture. Combining IL-12 and IL-15, however, resulted in decreased CB CD3() CD8(+) expression compared with IL-15 alone. The percentage of CD3(+) CD25(+) cells in CB MNCs spontaneously increased in the absence of cytokines,while that of CD3(+) CD56(+) cells in CB MNCs could not be enhanced with cytokines. In contrast, the percentages of CD3(+) CD25(+) and CD3(+) CD56(+)cells among the APE MNCs could be increased with IL-12, IL-15, and furtherwith IL-12 and IL-15 combined. Thus, different patterns of T-cell subset changes were demonstrated between CB MNCs and APE MNCs in response to IL-12 and/or IL-15. These data may serve as a foundation for using cytokine therapy in newborns and children receiving CB transplants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:17:21