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Titolo:
In vitro inhibition of Chlamydia trachomatis growth by liposome-encapsulated cyclines.
Autore:
Sangare, L; Morisset, R; Ravaoarinoro, M;
Indirizzi:
Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ H3C 3J7, Canada UnivMontreal Montreal PQ Canada H3C 3J7 ol, Montreal, PQ H3C 3J7, Canada CHUM, Dept Microbiol & Infectiol, Montreal, PQ H2W 1T8, Canada CHUM Montreal PQ Canada H2W 1T8 & Infectiol, Montreal, PQ H2W 1T8, Canada
Titolo Testata:
PATHOLOGIE BIOLOGIE
fascicolo: 1, volume: 49, anno: 2001,
pagine: 53 - 56
SICI:
0369-8114(200102)49:1<53:IVIOCT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
FRE
Soggetto:
TETRACYCLINE; ERYTHROMYCIN; INFECTIONS; RESISTANT; CARRIERS;
Keywords:
Chlamydia trachomatis; doxycycline; liposome; tetracycline;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Sangare, L Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ H3C 3J7, Canada Univ Montreal Montreal PQ Canada H3C 3J7 l, PQ H3C 3J7, Canada
Citazione:
L. Sangare et al., "In vitro inhibition of Chlamydia trachomatis growth by liposome-encapsulated cyclines.", PATH BIOL, 49(1), 2001, pp. 53-56

Abstract

The antichlamydial activity of tetracycline (Tet) and doxycycline (Dox) encapsulated in cationic (CaL), anionic (AnL) and neutral (NtL) liposomes hasbeen evaluated in vitro by adding serial dilutions of antibiotics (minimuminhibitory concentration, MlC: 0.12-0.007 mug/ml; MBC: 4 to 0.25 mug/mi) to HeLa 229 cell monolayers inoculated with Chlamydia trachomatis L-2/434/Bu(10(3) ufi/ml). Following 72 h incubation at 37 degrees C under a 5% CO2 atmosphere, the chlamydial inclusions were stained by the May-Giemsa method to determine the MICs. After a second and third passage, the MBC1 and MBC2 were determined in antibiotic-free medium. The chlamydial inclusions were then counted to assess the degree of growth inhibition at each antibiotic dilution tested for MBC1 and MBC2 determinations. The MlC, MBG(1) and MBC2 ofthe various antichlamydial agents were as follows. Tet (0.12; 4; 4), AnL-Tet (0.01; 1; 1), NtL-Tet (0.03; 1; 2), Dox (0.06; 1; 2), CaL-Dox (0.03; 0.5; 2), AnL-Dox (0.01; 1; 2), and NtL-Dox (0.03; 0.5; 0.5). It was found thatTet and Dox liposome-encapsulated antibiotics were more active than their non-encapsulated counterparts, and the inclusion count showed a higher inhibitory activity of the former antibiotics on chlamydial growth. The inhibition of chlamydial growth by AnL-Tet may be of bactericidal nature. In conclusion, liposome-encapsulated drugs could be of value in the treatment of chlamydial infections (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 00:53:52