Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of secretory and morphologic properties of primary cultured endocrine cells from porcine pancreata
Autore:
Niwa, T; Fukasawa, T; Yu, W; Nimura, Y; Senda, T; Ohgawara, H; Niki, I;
Indirizzi:
Nagoya Univ, Sch Med, Dept Surg 1, Nagoya, Aichi 466, Japan Nagoya Univ Nagoya Aichi Japan 466 Dept Surg 1, Nagoya, Aichi 466, Japan Nagoya Univ, Sch Med, Dept Cell Pharmacol, Nagoya, Aichi 466, Japan NagoyaUniv Nagoya Aichi Japan 466 ll Pharmacol, Nagoya, Aichi 466, Japan Nagoya Univ, Sch Med, Dept Anat 1, Nagoya, Aichi 466, Japan Nagoya Univ Nagoya Aichi Japan 466 Dept Anat 1, Nagoya, Aichi 466, Japan Tokyo Womens Med Univ, Inst Med Res, Sch Med, Tokyo, Japan Tokyo Womens Med Univ Tokyo Japan , Inst Med Res, Sch Med, Tokyo, Japan
Titolo Testata:
PANCREAS
fascicolo: 2, volume: 22, anno: 2001,
pagine: 135 - 140
SICI:
0885-3177(200103)22:2<135:COSAMP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN-SECRETION; IN-VITRO; ISLETS; XENOTRANSPLANTATION;
Keywords:
porcine islet cells; insulin release; glucagon-like peptide-1; sulfonylureas; autolytic beta-cell purification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Niki, I Nagoya Univ, Sch Med, Dept Pharmacol, 65 Tsuruma Cho, Nagoya, Aichi 4668550, Japan Nagoya Univ 65 Tsuruma Cho Nagoya Aichi Japan 4668550 68550, Japan
Citazione:
T. Niwa et al., "Characterization of secretory and morphologic properties of primary cultured endocrine cells from porcine pancreata", PANCREAS, 22(2), 2001, pp. 135-140

Abstract

We characterized morphologic and secretory properties of porcine pancreatic endocrine cells in primary culture obtained by autolytic preparation without any exogenous proteolytic enzymes. The endocrine cells exhibited a neuron-like shape, and insulin granules were accumulated at the terminal of theprocesses. Thus derived endocrine cells survived in culture medium containing nicotinamide and remained sensitive to glucose for at least 6 weeks after preparations. The cells responded well to physiologic concentrations of glucose, and high K+ depolarization and the antidiabetic sulfonylureas, tolbutamide, and glibenclamide also elicited the release. With high glucose, insulin release was markedly potentiated by forskolin, glucagon, glucagon-like peptide-1, and arginine and inhibited by somatostatin, the Ca2+ channel blocker nitrendipine, and the ATP-sensitive K+ channel opener diazoxide. Epinephrine had dual effects on the release by glucose; enhanced within a lownanomolar range and inhibited at 1 mu mol/L. However, the cells were unresponsive to leucine. Such secretory sensitivities to nutrients, hormones, and pharmacologic agents, and long survival rate las long as 5-6 weeks) of these cells suggest to us therefore that derived endocrine cells may be useful for xenotransplantation of pancreatic beta cells for treatment of insulin-dependent diabetes mellitus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 04:50:37