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Titolo:
Postsynaptic target regulates functional responses induced by 5-HT3 serotonin receptors on axonal varicosities of NG108-15 hybrid neuroblastoma cells
Autore:
Ronde, P; Nichols, RA;
Indirizzi:
Med Coll Penn & Hahnemann Univ, Dept Pharmacol & Physiol, Philadelphia, PA19102 USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA 19102 hia, PA19102 USA Med Coll Penn & Hahnemann Univ, Dept Neurobiol & Anat, Philadelphia, PA 19102 USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA 19102 ia, PA 19102 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 102, anno: 2001,
pagine: 979 - 987
SICI:
0306-4522(2001)102:4<979:PTRFRI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION-CHANNEL; SYNAPTIC TRANSMISSION; NERVE-TERMINALS; CA2+ CHANNELS; RAT STRIATUM; CALCIUM; MODULATION; RELEASE; BRAIN; 5-HYDROXYTRYPTAMINE;
Keywords:
presynaptic receptors; calcium; confocal imaging; nerve-muscle co-cultures;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Nichols, RA Med Coll Penn & Hahnemann Univ, Dept Pharmacol & Physiol, Philadelphia, PA19102 USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA 19102 USA
Citazione:
P. Ronde e R.A. Nichols, "Postsynaptic target regulates functional responses induced by 5-HT3 serotonin receptors on axonal varicosities of NG108-15 hybrid neuroblastoma cells", NEUROSCIENC, 102(4), 2001, pp. 979-987

Abstract

Rat brain presynaptic 5-HT3 serotonin receptors. members of the ligand-gated ion channel superfamily, induce changes in nerve terminal [Ca2+](i) in amanner distinct from that found for somatic 5-HT3 receptors. Here, we assessed the role of postsynaptic target in regulating the nature of presynaptic receptor-induced responses, using the hybrid neuroblastoma cell line NG108-15 as a model neuronal system that expresses 5-HT3 receptors. Using immunocytochemistry, 5-HT3 receptors were found to be present on the presynaptic-like varicosities of differentiated NG108-15 cells, indicating that these receptors possess an inherent ability to localize to potential presynaptic sites. In the absence of postsynaptic target, 5-HT3 receptors localized to the varicosities induce rapid but transient changes in [Ca2+](i) that were initiated by voltage-gated Ca2+ channels, as assessed using Ca2+ channel blockers, these properties being typical of those found for somatic 5-HT3 receptors. In co-cultures containing rat myotubes, with which NG108-15 cells form functional cholinergic synapses, the 5-HT3 receptor-induced changes in [Ca2+](i) in the axonal varicosities shifted over time (three to 10 days) to that found for brain nerve endings: sustained responses that were insensitive to blockade by antagonists of voltage-gated Ca2+ channels. The effect of co-culturing myotubes with the NG108-15 cells was mimicked by conditioned media from myotube cultures. These results indicate that regulatory molecules from the target postsynaptic cell dictate the functional responses elicited by presynaptic 5-HT3 receptors. Because the target-induced changes required several days before they were evident, we hypothesize that changes in protein expression, perhaps the consequence of altered gene regulation, underlie the changes in the responses to 5-HT3 receptor activation in the axonal varicosities of this neuronal cell line. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:41:21