Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat
Autore:
Ruhl, A; Trotter, J; Stremmel, W;
Indirizzi:
Univ Heidelberg, Dept Gastroenterol, Heidelberg, Germany Univ Heidelberg Heidelberg Germany t Gastroenterol, Heidelberg, Germany Univ Heidelberg, Dept Neurobiol, D-6900 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-6900 ol, D-6900 Heidelberg, Germany
Titolo Testata:
NEUROGASTROENTEROLOGY AND MOTILITY
fascicolo: 1, volume: 13, anno: 2001,
pagine: 95 - 106
SICI:
1350-1925(200102)13:1<95:IOEGAE>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEU TYROSINE KINASE; NERVOUS-SYSTEM; SCHWANN-CELLS; GUINEA-PIG; AUERBACHS PLEXUS; MONOCLONAL-ANTIBODIES; NEURONAL ELEMENTS; CHIMERIC RECEPTOR; PERIPHERAL-NERVE; TISSUE-CULTURE;
Keywords:
cell lines; enteric glia; rat; tissue culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Ruhl, A Univ Klinikum, Bergheimer Str 58, D-69115 Heidelberg, Germany UnivKlinikum Bergheimer Str 58 Heidelberg Germany D-69115 ermany
Citazione:
A. Ruhl et al., "Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of adult rat", NEUROG MOT, 13(1), 2001, pp. 95-106

Abstract

Although enteroglial cells (EGCs) may play a key role in the inflammatory response of the enteric nervous system, little is known about their immunophysiological properties. To facilitate further characterization of enteric glia, we have developed a novel method to isolate and purify EGCs from the myenteric plexus. Myenteric plexus preparations were enzymatically dissociated and EGCs purified by complement-mediated cytolysis of contaminating cells and transformed by retroviral gene transfer. Primary and transformed cells were characterized immunohistochemically and by dot-blot analysis. Functionally, c-fos mRNA expression was assessed in primary and transformed enteroglial cells. All cells displayed robust glial fibrillary acidic protein, S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha -actin or C3 complement receptor immunoreactivity. This confirmed their enteroglial lineage and excluded contamination with other cell types. Both primary and transformed EGCs displayed little constitutive c-fos mRNA expression. This, however, could be upregulated by various stimuli, including proinflammatory cytokines. In summary, we present a novel method to purify EGCs from rat myenteric plexus for tissue culture and to establish transformed EGC lines that retain their glial nature and functional properties. Such cell lines are now available for physiological studies of the functional properties of enteric glia in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:26:31