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Titolo:
IL-1 beta and IL-10 have dual effects on enteric glial cell proliferation
Autore:
Ruhl, A; Franzke, S; Stremmel, W;
Indirizzi:
Univ Heidelberg, Dept Gastroenterol, Heidelberg, Germany Univ Heidelberg Heidelberg Germany t Gastroenterol, Heidelberg, Germany
Titolo Testata:
NEUROGASTROENTEROLOGY AND MOTILITY
fascicolo: 1, volume: 13, anno: 2001,
pagine: 89 - 94
SICI:
1350-1925(200102)13:1<89:IBAIHD>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY BOWEL-DISEASE; CENTRAL-NERVOUS-SYSTEM; NEU TYROSINE KINASE; INTERLEUKIN-10; CYTOKINES; BRAIN; ASTROGLIOSIS; ASTROCYTES; NECROSIS; RECEPTOR;
Keywords:
cytokines; enteric glial cells; in vitro; proliferation; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ruhl, A Univ Klinikum, Bergheimer Str 58, D-69115 Heidelberg, Germany UnivKlinikum Bergheimer Str 58 Heidelberg Germany D-69115 ermany
Citazione:
A. Ruhl et al., "IL-1 beta and IL-10 have dual effects on enteric glial cell proliferation", NEUROG MOT, 13(1), 2001, pp. 89-94

Abstract

Inflammatory bowel disease is typically accompanied by functional and structural changes of the enteric nervous system. In pathological studies, cellular loss and axonal degeneration have been described in the myenteric plexus. However, more recent studies suggest that the proliferation rate of myenteric glial cells is enhanced in animal models of intestinal inflammation. Therefore, we have investigated the effect of different cytokines on the proliferative response of enteric glial cells (EGCs), comparing transformed enteric glial cell lines, primary astrocyte cultures and transformed oligodendrocytes. Cells were incubated in serum-free chemically defined medium inthe presence or absence of either interleukin (IL)-1 beta or IL-10 at concentrations ranging between 0.1 and 100 ng mL(-1) for 48 h. Subsequently, [H-3]thymidine was added to each culture dish for an additional 6 h, and the amount of incorporated [H-3] was assessed. IL-1 beta significantly and dose-dependently suppressed [H-3]-uptake by EGCs. In contrast, IL-10 induced a biphasic response; IL-10 at low concentrations (0.1 ng mL(-1)) caused a significant suppression of [H-3]-uptake, whereas high concentrations (5-100 ngmL(-1)) significantly enhanced [H-3] uptake. These results indicate that EGC proliferation can be modulated by cytokines. The differential effects ofIL-1 beta and IL-10 suggest that during intestinal inflammation there may be a regulatory interplay between different classes of cytokines modulatingEGC proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 04:19:10